5,5-二乙烯基-2,2-联吡啶:可能的新型锌离子荧光探针(英文)  

5,5-Divinyl-2,2-bipyridine:a Possible New Prototype of Fluorescent Probe for Sensing Zn^(2+)

作　　者：韩焕美[1] 吴亚光[1] 肖守军[1] 

机构地区：[1]南京大学配位化学国家重点实验室南京大学化学化工学院,南京210093

出　　处：《无机化学学报》2010年第8期1426-1430,共5页Chinese Journal of Inorganic Chemistry

基　　金：江苏省青蓝工程资助项目

摘　　要：生物体系里微量元素锌在发育、新陈代谢和疾病的发生等多个领域扮演了重要的角色,定性和定量测量锌的含量将帮助我们理解锌的生物学意义。我们偶然发现5,5-二乙烯基-2,2-联吡啶(DVBP)在二氧六环里对锌离子呈现出类似比例计量型荧光探针的特性:即有2个荧光发射峰而且长波长峰随锌离子的增加按比例增长。但DVBP难溶于水而且2个荧光发射峰距离太近从而相互干扰,我们尝试利用硅氢反应将DVBP固定在多孔硅上制备感应锌离子的光极,我们发现多孔硅固相载体上的联吡啶通过与锌离子的螯合后荧光增强约8倍,比DVBP在溶液里与锌离子螯合后的荧光增强(约4.5倍)还要多,联吡啶对锌离子的荧光响应是一个值得继续探索的领域。In biological systems,zinc-ion plays an important role in growth,metabolism,disease occurrence and other aspects. Qualitative and quantitative measurements of zinc-ion will help us understand the biological significance of zinc-ion. We incidentally found that 5,5-divinyl-2,2-bipyridine（DVBP） in 1,4-dioxane presented a ratiometric sensing feature toward Zn^2＋,which possesses dual fluorescent emissions and the intensity of the long-wavelength emission increases ratiometricly with increasing the concentration of Zn^2＋. However the non-solubility of DVBP in aqueous media and its limitation for practical applications with the two emission bands overlapped severely render us to search for alternative sensing approaches. We immobilized DVBP on porous silicon （PS） supporting matrices for fabrication of Zn^2＋-sensing optode. We found that Zn^2＋ even enhanced the bipyridyl fluorescence ～8 times,stronger than its enhancement of DVBP＇s fluorescence（～4.5 times） in solution. Further research on fluorescent response of bipyridine derivatives to Zn^2＋ should be continued.

关 键 词：联吡啶 锌离子 荧光探针 多孔硅 

分 类 号：O614.241[理学—无机化学] TQ127.2[理学—化学]