耐盐杂草稻3个锌指蛋白基因家族的实时定量分析  

Real-Time Quantitative Analysis on Three Zinc Finger Protein Gene Family of Salt-Tolerant Weedy Rice (Oryza sativa f.spontanea)

作　　者：张丽丽[1] 孙健[1] 马殿荣[1] 陈温福[1] 

机构地区：[1]沈阳农业大学水稻研究所,农业部北方作物生理生态重点开放实验室,辽宁省北方粳稻遗传育种重点实验室,沈阳110866

出　　处：《植物生理学通讯》2010年第6期529-536,共8页Plant Physiology Communications

基　　金：国家自然科学基金项目(30671262);教育部高等学校博士学科点专项科研基金项目(20060157003);水稻生物学国家重点实验室开放课题

摘　　要：利用在300余份来源于辽宁、吉林、黑龙江、内蒙古、江苏等地的杂草稻材料中筛选出耐高盐杂草稻材料WR03-12。通过RT-PCR的方法得到盐胁迫下WR03-12与盐敏感栽培稻‘越光’幼苗的cDNA第一链,对3个锌指蛋白基因家族的6个基因表达情况进行了荧光实时定量分析。结果表明,2个C2C2型锌指蛋白基因SRZ1与SRZ2受到高盐胁迫的负向诱导,WR03-12受负向诱导程度要小于‘越光’;2个TFIIIA型锌指蛋白基因ZFP18与ZFP245受到盐胁迫的正向诱导,WR03-12受诱导程度也小于‘越光’;具有A20锌指结构的基因AACZ1基因在越光中不受盐诱导,而在WR03-12中受短时间诱导后,第7天已经恢复到胁迫前水平。具有AN1锌指结构的基因AACZ2在‘越光’与WR03-12中均不受盐胁迫诱导,且表达水平没有显著差别。杂草稻WR03-12与‘越光’对于盐胁迫的应答机制可能在转录调控方面存在差别。Salt-torlerant weedy rice WR03-12 was screend from 300 accessions derived from Liaoning,Jilin,Heilongjiang,Inner Mongolia and Jiangsu Province.To investigete the salt-tolerant mechanism of WR03-12 in transcriptional regulation level,the first strand cDNA of salt-sensitive varietiy ＇Koshihikari＇ and salt-tolerant weedy rice WR03-12 were obtained,and the expression of six gene in three zinc finger protein family of the two germplasm was studied by Real-time quantitative PCR.Results showed （i） the C2C2 pattern gene of SRZ1 and SRZ2 was induced negatively by high salt stress,and the expression level in WR03-12 was lower than ＇Koshihikari＇;（ii） the TFIIIA pattern gene of ZFP18 and ZFP245 was induced positively by high salt stress,and the expression level in WR03-12 was lower than ＇Koshihikari＇;（iii） the expression of A20 pattern gene AACZ1 was not induced in ＇Koshihikari＇,while was induced in a short time （7 day） in WR03-12 under high salt stress;（iv） the expression of AN1 pattern gene AACZ2 was not induced by high salt stress in both ＇Koshihikari＇ and WR03-12.Results suggested that the salt stress response mechanism between WR03-12 and ＇Koshihikari＇ may be different in transcriptional regulation.

关 键 词：杂草稻 锌指蛋白 转录调控 实时定量PCR 

分 类 号：S511[农业科学—作物学] Q943[生物学—植物学]