拟南芥中光信号系统中关键基因CRY1、CRY2和COP1启动子的表达模式分析  

Analysis of Expression Patterns of CRY1,CRY2 and COP1 Promoters in Arabidopsis thaliana

作　　者：汤淼[1] 杨洪全[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《植物生理学通讯》2010年第6期541-548,共8页Plant Physiology Communications

基　　金：国家自然科学基金(30830012);中国科技部基金(2006AA10A102);国家转基因作物专项基金(2009ZX08009-081B)

摘　　要：通过构建表达光信号系统关键基因CRY1、CRY2和COP1启动子与GUS融合基因的拟南芥转基因植株,并对转基因植株进行GUS组织化学染色的结果表明,CRY1、CRY2和COP1的表达模式不受光条件的调控,并且在各器官有广泛的表达。分别分析CRY1基因启动子在cop1突变体以及COP1基因启动子在cry1突变体遗传背景中表达模式的结果表明,CRY1和COP1在转录水平上不存在明显的相互调控关系。Based on GUS histological staining results,we demonstrated that the expression of three key genes in light signalling pathway,namely CRY1,CRY2 and COP1,were not regulated by light conditions in Arabidopsis thaliana.We also showed that CRY1,CRY2 and COP1 were extensivly expressed in all organs of grown plants.By analysing the expression pattern of CRY1 in cop1 mutant,and COP1 in cry1 mutant,we concluded that these two genes may not regulate each other at the transcriptional level.

关 键 词：隐花色素 COP1 GUS染色 表达模式 拟南芥 

分 类 号：Q943.2[生物学—植物学]