紫外辐射诱导烟草原生质体中DNA损伤的彗星电泳检测  

Comet Assay on DNA Damage Induced by UV-B Radiation in Tobacco Protoplasts

作　　者：张来军[1,2] 郝建国[1] 贾敬芬[1] 

机构地区：[1]西北大学西部资源生物与现代生物技术教育部重点实验室,西安710069 [2]甘肃省陇东学院生命科学系,甘肃庆阳745000

出　　处：《植物生理学通讯》2010年第6期597-600,共4页Plant Physiology Communications

基　　金：国家自然科学基金(30671082)

摘　　要：以烟草原生质体为材料,采用彗星电泳检测用0.5W·m-2紫外线以不同时间(0、5、10、30、60和120s)诱导的烟草原生质体中DNA的损伤。结果表明,在0～10s的时间内代表DNA损伤程度的尾矩、Olive尾矩等参数与紫外线照射时间具有良好的时间依赖关系。本文建立的烟草原生质体体系采用彗星电泳技术,可以快速而灵敏地检测紫外线对植物细胞的损伤程度。Protoplasts of Nicotiana sylvestris was used for experimental material to investigate DNA damage in protoplast which was induced by 0.5 W·cm^-2 UV-B for different time （0,5,10,30,60,and 120 s） by using comet assay.The results showed that the indexes of tail moment and olive tail moment etc which detected the DNA damage had statistically dose effect relationships with the time of UV-B radiation from 0 to 10 s.Comet assay detected the degree of DNA damage of tobacco protoplast induced by UV-B radiation rapidly and sensitively.

关 键 词：彗星电泳 DNA损伤 UV-B辐射 烟草原生质体 

分 类 号：S572[农业科学—烟草工业]