An efficient synthesis of ampicillin on magnetically separable immobilized penicillin G acylase  

作　　者：Ping Xue Xiao Dan Song Xue Rong Cao 

机构地区：[1]Key Laboratory of Energy Resources&Chemical Engineering,Ningxia University,Yinchuan 750021,China

出　　处：《Chinese Chemical Letters》2010年第7期765-768,共4页中国化学快报(英文版)

基　　金：supported by The National Basic Research Program 973 of China(No.2010CB534916);the National Natural Science Foundation of China(Nos.50662004,20564002).

摘　　要：Penicillin G acylase(PGA) was immobilized on the magnetic hydrophilic polymer microspheres with average pore size of 17.1 nm,specific surface area of 128.2 m2/g and saturate magnetization of 6.4 emu/g.The 96.7%ampicillin yield with 1.60 of the synthesis/hydrolysis(S/H) ratio from 6-aminopenicillanic acid(6-APA) and D-(-)-alpha-phenylglycine methyl ester(D-PGME) can be achieved using the resultant magnetic biocatalyst in ethylene glycol,where only 82.1%yield with 1.40 of the S/H ratio was obtained using the free PGA under the identical reaction conditions.The immobilized PGA can be separated magnetically and recycled for five times without obvious loss of its catalytic activity.Penicillin G acylase(PGA) was immobilized on the magnetic hydrophilic polymer microspheres with average pore size of 17.1 nm,specific surface area of 128.2 m2/g and saturate magnetization of 6.4 emu/g.The 96.7%ampicillin yield with 1.60 of the synthesis/hydrolysis(S/H) ratio from 6-aminopenicillanic acid(6-APA) and D-(-)-alpha-phenylglycine methyl ester(D-PGME) can be achieved using the resultant magnetic biocatalyst in ethylene glycol,where only 82.1%yield with 1.40 of the S/H ratio was obtained using the free PGA under the identical reaction conditions.The immobilized PGA can be separated magnetically and recycled for five times without obvious loss of its catalytic activity.

关 键 词：Immobilized penicillin G acylase Magnetic polymer microspheres Ampicillin synthesis REUSABILITY 

分 类 号：O621.2[理学—有机化学]