CD41标记的微小巨核细胞染色在老年贫血诊断中的应用  

Application of CD41 labelled micromegakaryocyte staining in differential diagnosis of senile anemia

作　　者：邢颖[1] 曲妍[1] 黄佳玲[1] 林树东[1] 

机构地区：[1]齐齐哈尔医学院附属第三医院检验科,黑龙江齐齐哈尔161000

出　　处：《东南大学学报（医学版）》2010年第4期449-452,共4页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:探讨CD41标记的微小巨核细胞染色法(SAP染色法)在老年贫血诊断及鉴别诊断中的意义。方法:采用瑞特-姬姆萨染色和CD41标记的SAP染色法,分别检测了107例60岁以上老年贫血患者的骨髓涂片中微小巨核细胞的数量和类型。结果:CD41标记的SAP染色法对巨核细胞的检出率明显高于瑞特-姬姆萨染色法,两者相比差异有统计学意义(P<0.01)。不同疾病组比较,微小巨核细胞阳性率MDS组最高,依次为MDS>ITP>MA>ACD>MM>IDA>RA>AA>PRCA>CAA;淋巴样微小巨核细胞MDS组检出率为73.6%,其他组均未检出。结论:CD41标记的SAP染色可以显著提高巨核细胞检出率;且对老年贫血具有一定的鉴别诊断价值。Objective：To assess the significance of the CD41 labelled micromegakaryocyte Streptavidin-Alkaline Phosphatase（SAP） staining in the diagnosis and differential diagnosis of senile anemia.Methods：Bone marrow smears from 107 patients with anemia over the age of 60 were detected by Wright-Giemsa staining and CD41 labelled SAP staining for the number and types of micromegakaryocytes,respectively.Results：The detection rates of megakaryocyte by the CD41 labelled SAP staining and Wright-Giemsa staining had significant difference（P 0.01）.Among the groups,MDS group for the positive rate of micromegakaryocyte ranked the first,followed by ITP MA ACD MM IDA RA AA PRCA CAA;Detection rate of lymphoid micromegakaryocyte in the MDS group was 73.6%,there were not micromegakaryocytes observed in other groups.Conclusion：CD41 labelled SAP staining obviously increases the detection rate of megakaryocyte,which is valuable for differential diagnosis of senile anemia.

关 键 词：CD41单抗 微小巨核细胞 SAP染色 老年贫血 鉴别诊断 

分 类 号：R556[医药卫生—血液循环系统疾病] R446[医药卫生—内科学]