SLE患者外周血Vγ9链T细胞受体CDR3特征  

The characteristics of CDR3 in Vγ9 T cell receptor from human peripheral blood in patients with systemic lupus erythematosus

作　　者：王丽芳[1,2] 康宁[1,2] 崔莲仙[1,2] 巴德年[1,2] 何维[1,2] 

机构地区：[1]中国医学科学院基础医学研究所 [2]北京协和医学院基础学院免疫学系,北京100005

出　　处：《现代免疫学》2010年第4期265-270,共6页Current Immunology

基　　金：国家重点基础研究发展计划(973计划)(2007CB512405)

摘　　要：为探讨Vγ9链T细胞受体(Tcell receptor,TCR)互补决定区3(complementarity determining region3,CDR3)是否参与自身免疫性疾病中γδT细胞的抗原识别过程,利用基因扫描技术和随机序列测定法对系统性红斑狼疮(systemic lupus er-ythematosus,SLE)患者和健康人外周血Vγ9TCR CDR3区的结构特征进行分析。结果显示,Vγ9CDR3序列的变化主要集中在J区等位基因的取用不同:健康人以表达JγP为主(82.86%),而SLE患者Jγ1/Jγ2(40.82%)和JγP(59.18%)表达率相近。两组人群相比,Jγ1/Jγ2和JγP表达率的差异均具有显著性。结果表明,SLE患者外周血Vγ9链T细胞受体具有与健康人不同的CDR3组成特点,提示其可能参与SLE病理过程中γδT细胞的抗原识别。本研究为寻找SLE中γδT细胞识别的自身抗原以及阐明γδT细胞在SLE中的独特作用奠定了基础。To determine whether complementarity determining region 3 （CDR3） of T cell receptor （TCR） Vγ9 chain is involved in the antigen recognition process of γδT cells in autoimmune disease,structural characteristics of Vγ9 TCR CDR3 were compared between patients with systemic lupus erythematosus （SLE） and healthy donors by size spectral analysis and random sequencing techniques. It was found that dominant Jγ usage of Vγ9 CDR3 was different between patients with SLE and healthy donors. In normal individuals,JγP was dominant （82.86%）,while in patients with SLE both Jγ1/Jγ2 （40.82%） and JγP （59.18%） were commonly seen. Both of these changes were significantly different in two groups. Therefore,Vγ9 TCR CDR3 characteristics were different between patients with SLE and healthy donors,suggesting that it may participate in the antigen recognition process of γδT cells in patients with SLE. Our results lay a foundation for finding autoantigens recognized by γδT cells and for elucidating the special roles that γδT cells played in the SLE status.

关 键 词：系统性红斑狼疮 ΓΔT细胞 T细胞受体 Vγ9链 互补决定区3 

分 类 号：R392.1[医药卫生—免疫学]