芸香甙对环孢素A肾毒性防护作用的实验研究  

Protection of Rutoside on nephrotoxicity caused by cyclosporine A in rats

作　　者：邱伟彬[1] 陈仙[1] 张彪[1] 丁军[1] 

机构地区：[1]南华大学第二附属医院,湖南衡阳421001

出　　处：《现代生物医学进展》2010年第13期2428-2431,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨芸香甙(Rutoside,Ru)对环孢素A(cyclosporine A,CsA)肾毒性防护作用及其机制。方法:取雄性SD大鼠20只,随机分为4组(n=5):正常对照组、Ru组、CsA模型组、CsA+Ru治疗组。CsA模型组和CsA+Ru治疗组均用CsA50mg/kg灌胃,Ru组与CsA+Ru组分别腹腔注射NS10ml/kg+Ru20mg/kg。以上各组每天给药一次,连续给药15天。各组大鼠于给药第14天后置代谢笼中收集24h尿液,测定尿Cr、尿蛋白含量。末次给药5个小时后,取血检测血清Cr、BUN含量和肾组织中丙二醛(MDA)含量、内皮素(ET)含量和超氧化物歧化酶(SOD)活性;肾组织用10%甲醛溶液固定,石蜡包埋,HE染色,光镜观察肾组织其形态学变化。结果:Ru对CsA所致的尿蛋白、BUN、Cr、肾组织MDA、ET含量的升高均有显著降低作用,并明显增加肾组织SOD活性,对CsA引起的肾小球与肾小管的病理性损伤有较好的保护作用。结论:Ru对CsA所致的肾脏毒性具有明显的保护作用。Objective：To explore the hypothesis and the mechanisms that Rutoside（Ru） may be protective against cyclosporine（CsA）-induced nephrotoxicity.Methods：Male Sprague-Dawley（SD） rats were randomly assigned into four groups of five rats each.Control group,Rutoside group,CsA group,CsA plus Rutoside group.CsA group and CsA plus Rutoside group were gived CsA（50 mg/kg） with Olive,Rutoside group and CsA plus Rutoside group were gived Rutoside（20mg/kg）with NS（10ml/kg）by Intraperitoneally.The all groups were administered once day respectively for 15 days.Twenty-four hour urines were collected at the 14th day respectively after administration to measure the content of urinary protein and urinary creatinine（UCr）.The rats in all groups were anaesthetized 5 h after the last administration,Blood samples were collected to measure serum creatinine（SCr）,and blood urea nitrogen（BUN）.Kidneys were removed rapidly,excised and sectioned for histological analysis.Other kiney tissues were utilized for biochemical analysis.Result：Quercetin prevented urinary protein,SCr,BUN,and kidneys histological alterations.Rutoside significantly decreased malondial dehyde（MDA） and endothelin（ET） content while significantly increased,superoxide dismutase（SOD） activities of kidneys tissues in therats treated with CsA.Conclusion：Rutoside can effectively attenuate the nephrotoxicity induced by CsA.

关 键 词：芸香甙 环孢素肾毒性 超氧化物歧化酶 丙二醛 内皮素 

分 类 号：S512.1[农业科学—作物学]