强德勒红心柚离体培养与植株再生体系的建立  

Establishment for Culture In Vitro and Plant Regeneration System of Citrus grandis cv. Chandler

作　　者：曾艳[1,2] 苏智先[2] 邹利娟[2] 吴庆贵[2] 毛艳萍[1,2] 程昌凤[3] 

机构地区：[1]西华师范大学生命科学学院,四川南充637002 [2]四川省生态与环境技术重点实验室,四川绵阳621000 [3]重庆市农业科学院果树研究所,重庆江津402260

出　　处：《武汉植物学研究》2010年第2期218-223,共6页Journal of Wuhan Botanical Research

基　　金：四川省教育厅项目(2003A175);四川省绵阳市科技攻关项目(2005BN001-1)

摘　　要：以强德勒红心柚(Citrus grandis Osbeckcv. Chandler)种子萌发的无菌苗为材料,选取子叶、子叶节段、上胚轴、带芽的茎段进行离体培养研究。结果表明:子叶节段是诱导丛生芽的最佳外植体,诱导率100%;诱导丛生芽的最佳培养基为MS+6-BA2.0mg/L+NAA0.05mg/L+蔗糖30g/L+活性炭0.4g/L,丛生芽增殖可达11.2倍;最适生根培养基为1/2MS+NAA0.5mg/L,生根率达100%,移栽15d后成活率100%。The cotyledons,cotyledonary nodes,epicotyl segments,and stem segments with lateral buds of aseptic Citrus grandis Osbeck cv.Chandler seedings were used as the explants to study the process of tissue culture rapid reproduction.Results showed that the cotyledonary nodes were the best explants of bush-bud induction（induction rate 100%）.The best medium for inducing bush-bud was MS＋6-BA 2.0 mg/L＋NAA 0.05 mg/L＋sucrose 30 g/L＋activated carbon 0.4 g/L,the coefficient of proliferation reached 11.2.The optimum medium for shoot rooting was 1/2MS＋NAA 0.5 mg/L,which resulted in a rooting rate of 100%.Fifteen days after transplanting,100% of the plantlets survived.

关 键 词：强德勒红心柚 离体培养 丛生芽增殖 

分 类 号：Q943.1[生物学—植物学] S666.3[农业科学—果树学]