膜结合型巨噬细胞集落刺激因子对巨噬细胞功能性极化影响的探索  

Study on the effects of mM-CSF in the functional polarization of macrophages

作　　者：许琳琳[1] 郑国光[1] 马翠花[1] 贾海蓉[1] 种靖慧[1] 刘淑艳[1] 林永敏[1] 

机构地区：[1]中国医学科学院北京协和医学院血液学研究所血液病医院实验血液学国家重点实验室,天津300020

出　　处：《白血病．淋巴瘤》2010年第7期388-390,共3页Journal of Leukemia & Lymphoma

摘　　要：目的 探讨高表达膜结合型巨噬细胞集落刺激因子（mM-CSF）的血液肿瘤细胞对巨噬细胞的异常活化作用.方法 采用稳定表达mM-CSF的淋巴瘤细胞系Namalwa-M和巨噬细胞系RAW264.7体外共培养体系,以转入空载体的Namalwa-V细胞系共培养体系为对照组.流式细胞术检测RAW264.7的CD206（选择性活化巨噬细胞高表达的表面分子）和细胞内白细胞介素（IL）-6、IL-10、IL-12及TNF-α的表达水平;墨汁吞噬实验检测共培养RAW264.7细胞的功能变化.结果 与Namalwa-M共培养的RAW264.7的CD206表达水平明显升高平均均荧光强度为55.12±3.77,提示其巨噬细胞活性增强;IL.10、TNF-α表达上调（201±6）%、（136±6）%;IL-12、IL-6表达下调（990±528）%、（60 ±26）%;吞噬能力明显提高.结论 高表达mM-CSF的血液肿瘤细胞可异常活化巨噬细胞成为高表达IL-10、TNF-α,低表达IL-12,IL-6的异常活化巨噬细胞.Objective To investigate the anti-inflammatory/immune modulatory effects of high-expressing membrane bound M-CSF-hematopoietic malignant cells on macrophages. Methods After coculturing RAW264.7 and murine macrophage cell line with Namalwa-M, a cell line stably expressing mM-CSF, and companing with Nainalwa-V, a cell line stably transfected with the empty vector as the control, flow cytometry was used to detect the expression of the marker of alternatively activated macrophages, CD206, and intracellular expression of IL-10, IL-12, IL-6 and TNF-α to study the immunophenotype of RAW264.7; phagocytic assays to investigate their functional activity in vitro. Results RAW264.7 cocultured with Namalwa-M consistently showed high-level expression of CD206, which indicated activities of these macrophage cells were increased. Furthermore, these RAW264.7 expressing high levels of IL-10, TNF-a and low levels of IL-12, IL-6, as determined by intracellular staining were suggested that the phagocytic activity was increased. Functionally, RAW264.7 cocultured with Namalwa-M showed a higher level of phagocytic activity. Conclusion Macrophage generated in vitro after cocultured with mM-CSF-expressing hematopoietic malignant cell line could be transformed into abnormal macrophage with an immunophenotype defined as IL-10-high, IL-12-low, IL-6-low and TNF-α-high.

关 键 词：膜结合型巨噬细胞集落刺激因子 巨噬细胞 免疫细胞功能性极化 细胞因子 吞噬 

分 类 号：R392[医药卫生—免疫学]