水杨酸钠对百草枯所致大鼠肺组织核转录因子和炎性因子的影响  

Mechanism of Sodium Salicylate on Paraquat-induced Effects of NF-κB,TNF-α,and TGF-β_1 in Rat Lung

作　　者：俞爱青[1] 常秀丽[1] 黄敏[1] 张平[1] 吴庆[1] 顾锡安[1] 周志俊[1] 

机构地区：[1]复旦大学公共卫生学院,教育部公共卫生安全重点实验室,上海200032

出　　处：《环境与职业医学》2010年第7期394-397,共4页Journal of Environmental and Occupational Medicine

基　　金：国家科技支撑计划(编号:2006BAI06B01);上海市公共卫生学科建设项目(编号:08GWZX0303,08GWD20)

摘　　要：[目的]探讨水杨酸钠(sodium salicylate,NaSAL)对抗百草枯(paraquat,PQ)致肺损伤的作用机制。[方法]将72只雄性SD大鼠随机分为溶剂对照组(生理盐水灌胃)、PQ染毒组和PQ+NaSAL干预组。PQ经口染毒,剂量为80 mg/kg(按大鼠体重),干预组给予染毒组相等剂量PQ后立即腹腔注射NaSAL(120 mg/kg)。在染毒后第1、3、7、14天取对照组、染毒组、干预组各6只动物进行检查,分别用凝胶电泳迁移率实验(EMSA)和酶联免疫吸附法(ELISA)检测肺组织中核转录因子(NF-κB)活性变增高;肺组织中TNF-α、TGF-β1蛋白表达增强,各时点蛋白水平与对照组相比差异有统计学意义(P<0.05)。干预组NF-κB活性较染毒组降低;与染毒组相比,干预组肺组织TNF-α、TGF-β1蛋白水平表达明显下降,相应时点差异也有统计学意义(P<0.05)。[结论]NaSAL干预后可以降低肺组织中NF-κB的活性,减少TNF-α、TGF-β1的蛋化及肿瘤坏死因子(TNF-α)、转化生长因子-β(1TGF-β1)蛋白含量的改变。[结果]染毒组大鼠肺组织中NF-κB活性白表达,提示NaSAL可以改善PQ中毒引起的肺损伤。[Objective] To investigate the potential protective mechanism of sodium salicylate（NaSAL）on the pulmonary damage induced by paraquat（PQ）in rats.[Methods] We established rat poisoning model by intragastric administration of 80mg/kg PQ.A total of 72 male SD rats were randomly divided into control group,PQ treated group and PQ plus NaSAL intervention group.Control group were orally administered with an injection of 0.9% NaCl at the volume of 0.005 mL/g of body weight;PQ treated group were made 80 mg/kg PQ orally;PQ plus NaSAL group were given an intraperitoneal injection of 120 mg/kg NaSAL after an orally administration of 80 mg/kg PQ.Animals were sacrificed in batches on days 1,3,7,14 after experiment.Then,the level of nuclear factor of kappa B（NF-κB）was assayed by electrophoresis mobility shift assay（EMSA）,while the protein levels of tumor necrosis factor-α（TNF-α）,transformin growth factor-β1（TGF-β1）were determined by enzyme-linked immunosorbent assay（ELISA）.[Results] After 80 mg/kg PQ exposure,the levels of TGF-β1,TNF-α protein expression and the rat lung tissue NF-κB activity increased more remarkably than that of the control group（P 〈0.05）.In comparison with PQ treated group,with the treatment of NaSAL（120 mg/kg）,the level of TNF-α,TGF-β1 protein expression and the rat lung tissue NF-κB activity decreased significantly（P 〈0.05）and trended to normal values.[Conclusion] Treatment of NaSAL could decrease the level of TNF-α,TGF-β1 protein expression and the rat lung tissue NF-κB activity.It suggested that NaSAL could alleviate paraquat-induced pulmonary damage in rats to some extent.

关 键 词：水杨酸钠 百草枯 肺损伤 

分 类 号：R114[医药卫生—卫生毒理学]