紫稻(Oryza sativa L.)线粒体ATP合成酶atp6基因转录本RNA编辑  

Mitochondrial RNA Editing of ATPase atp6 Gene Transcripts of Yunnan Purple Rice(Oryza sativa L.)

作　　者：魏磊[1,2] 费震江[1] 丁毅[2] 

机构地区：[1]湖北省农业科学院粮食作物研究所,武汉430064 [2]武汉大学生命科学学院植物发育生物学教育部重点实验室,武汉430072

出　　处：《武汉植物学研究》2010年第3期251-256,共6页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金(30571143)

摘　　要：紫稻(Oryza sativa L.)细胞质雄性不育系紫稻A是本实验室构建的新型细胞质雄性不育系。本研究使用PCR、RT-PCR、DNA测序等技术,得到了紫稻细胞质雄性不育水稻不育系(樱香A)及其保持系(樱香B)线粒体atp6基因转录本cDNA序列。通过与基因组序列比对发现:樱香Aatp6cDNA序列中,没有发生RNA编辑;而樱香Batp6 cDNA序列中有16个编辑位点,在樱香B cDNA序列16个编辑位点位于15个密码子中,所编码的氨基酸均发生改变:在1003位点由C替换为T,导致原来编码谷氨酰胺密码子(CAA)成为终止密码子(TAA),保证atp6 mRNA编码一个正常的ATP6多肽;而由于没有发生RNA编辑,樱香A mRNA就不能翻译成正常的多肽。研究表明,RNA编辑在合成正常的ATP6多肽的过程中具有至关重要的作用,同时也说明RNA编辑可能与细胞质雄性不育相关。Base on the of construction of a new type of cytoplasmic male sterile line Zidao A（Oryza sativa L.）,we analyzed the transcript editing of mitochondrial ATP synthase subunit 6 gene（atp6） from CMS line and its maintainer line.With PCR,RT-PCR,complete nucleotide sequences were determined for the mitochondrial atp6 gene cDNA from two lines of Purple Rice Type rice：cytoplasmic male sterile line Ying xiang A and its maintainer line Yingxiang B.The atp6 transcript of Yingxiang A had no editing site and the transcript of Ying xiang B had 16 editing sites with changes affecting 15 amino acid residues of the protein product.The editing of the atp6 transcript from Yingxiang B changed a glutamine codon（CAA） into a termination codon（TAA）,shortening the protein of Ying xiang B to the ＂standard＂ size.The Ying xiang A transcript,which had no editing site and a＂normal＂ termination codon,cannot be translated to a normal protein.The results demonstrated the important role of RNA editing in the production of the functional ATP6 subunit and suggested that RNA editing may be associated with cytoplasmic male sterility.

关 键 词：Atp6基因 RNA编辑 细胞质雄性不育 线粒体 紫稻 

分 类 号：Q943[生物学—植物学] S511[农业科学—作物学]