人工诱导花鳗鲡卵巢发育成熟及相关激素和组织的作用  

Artificial induction maturation of marbled eel(Anguilla marmorata) and functions of related hormones and tissues

作　　者：林浩然[1,2] 齐鑫[1] 周雯伊[1] 尹绍武[1] 黄海[1,2] 张勇[2] 刘晓春[2] 陈国华[1] 

机构地区：[1]海南大学海洋学院暨热带生物资源教育部重点实验室,海南海口570228 [2]中山大学水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室,广东广州510275

出　　处：《水产学报》2010年第7期989-998,共10页Journal of Fisheries of China

基　　金：国家自然科学基金面上项目(30770283);国家"十一五"科技支撑计划重点项目(2007BAD29B03);海南省教育厅高等学校科学研究资助项目(Hjkj2008-22);广东省科技兴海(渔)项目(B200701A06)

摘　　要：采用多次肌肉注射黄体生成素释放激素(luteinizing hormone releasing hormone,LHRH-A2)和鲤脑垂体匀浆(carp pituitary extract,CPE)的方法诱导花鳗鲡卵巢发育成熟。在任意挑选的15尾实验组鱼中,5尾卵巢发育完全成熟,平均性腺系数(gonadosomatic index,GSI)为(20.9±5.04)%(最大达到27.3%);5尾卵巢发育至成熟期,平均GSI为(7.2±2.86)%;5尾卵巢发育至卵黄生成期,平均GSI为(2.32±1.5)%;而对照组的性腺停留在未发育的Ⅱ期[GSI为(1.18±0.39)%],这表明注射的激素能诱导雌性花鳗鲡性腺发育直到完全成熟。检测卵巢发育过程中脑垂体GtH的mRNA水平,血清中类固醇激素水平以及肝脏和消化道组织学变化。结果表明,脑垂体中GtHα、LHβ和FSHβ的mRNA水平随着卵巢的发育而逐渐增加,在卵巢发育成熟后下降,其中GtHα和LHβ的含量在卵巢发育成熟时虽比发育时期略有下降,但仍然呈现高表达量,而FSHβ在卵巢发育成熟时的表达量极低,几乎检测不到。血清雌二醇的含量在卵巢开始发育和卵黄生成期时的含量较高,随着卵巢的发育成熟而下降;睾酮的含量在卵巢发育成熟过程中有所增加,在卵巢发育成熟时明显升高。肝糖原过碘酸希夫反应染色(periodic acid shiff,PAS)染色结果显示随着卵巢的发育成熟,肝细胞中糖原颗粒逐渐减少,脂肪含量有所增加,肝糖原参与卵巢生成过程中的物质与能量代谢活动。胃壁和肠壁的组织学显示,随着卵巢发育成熟而出现不同程度的退化。In the present study,female marbled eel（Anguilla marmorata） were induced to develop ovarian maturation by repeated injection of carp pituitaries extract（CPE） and LHRH-A2.Five in the fifteen randomly selected injected eels were matured,and were in the completely mature stage with the mean GSI of （20.9±5.04）%（the max GSI was 27.3%）; five were in the full-grown stage with the mean GSI of （7.2±2.86）%; five were in the vitellogenic oocytes stage with mean GSI of （2.32±1.5）%;while the controls were in theⅡ stage with the mean GSI of （1.18±0.39）%.The result showed that eels could be induced completely mature by the exogenous hormone management.mRNA levels of pituitary GtH subunits,serum steriod levels,and histology of hepar and alimentary canal of eels in different development stages were examined.Quantitative realtime PCR revealed that mRNA expression levels of GtHα,LHβ and FSHβ subunits increased with the oocytes development and then decreased after the maturation of ovary.The GtHα and LHβ levels decreased with the maturation of ovary,but were still highly expressed.While the FSHβ levels were hardly to be detected in the matured eels.Serum E2 levels were high in the early stages and rapidly decreased with the development of the ovary; Serum T levels increased during the ovary development,especially at the mature stage.The PAS stain reaction of hepar showed that the glycogen decreased with the ovary development,and the lipid droplets increased significantly.This can be directly attributed to the fact that glycogen in hepatocytes was involved in metabolism of matter and energy in the development of ovary.The histology of stomach and intestine wall showed varying degrees of degeneration during the development of ovary.

关 键 词：花鳗鲡 人工诱导 卵巢 促性腺激素 类固醇激素 肝脏 消化道 

分 类 号：Q954.4[生物学—动物学] S917[农业科学—水产科学]