苦参碱体外诱导卵巢癌3AO细胞凋亡的研究  

Matrine induced apoptosis in ovarian cancer cell line 3AO

作　　者：汪光蓉[1] 张国元[1] 蒋红[2] 凡瞿明[1] 唐中[1] 

机构地区：[1]川北医学院附属医院检验科,南充637000 [2]川北医学院附属医院风湿免疫研究所,南充637000

出　　处：《中药药理与临床》2010年第3期20-22,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：四川卫生厅课题(03007)

摘　　要：目的:观察中药苦参碱对卵巢癌3AO细胞的增殖抑制作用。方法:应用不同浓度的苦参碱作用3AO细胞,分别用倒置显微镜观察细胞形态学改变;MTT法检测苦参碱对3AO细胞的增殖抑制作用;流式细胞仪检测苦参碱对3AO细胞凋亡的影响。结果:苦参碱具有抑制卵巢癌3AO细胞增殖的作用,并呈明显的时间-剂量依赖性;在苦参碱作用下,3AO细胞出现凋亡改变;FCM检测结果显示经苦参碱作用后,细胞的凋亡率增加,呈现时间-剂量依赖性。结论:苦参碱能诱导卵巢癌细胞凋亡,抑制卵巢癌细胞增殖。Objective：To study the effect of matrine on the apoptosis of ovarian cancer cell at different concentrations.Methods：Matrine at different concentrations was respectively used to cultivate 3AO in vitro.Cell proliferation was assessed by MTT assays.Morphological changes of apoptosis were observed with invert microscope.Cell cycle were examined by PI fluorescence flow cytometry （ FCM） respectively.Results：Inhibition of matrine on the growth of 3AO cell was increasing with passing of time and increasing of concentration.Under the influence of matrine,3AO cell exhibited changes of apoptosis in morphology of microscope.Conclusion：matrine can inhibit cell proliferation and induces apoptosis in ovarian cancer cell line.

关 键 词：苦参碱 增殖 凋亡 卵巢癌 

分 类 号：R285[医药卫生—中药学] R737.31[医药卫生—中医学]