血小板活化及其相关炎症因子在儿童伴有喘息症状肺炎中的作用  

Role of platelet activation and platelet-related inflammatory factor in pneumonia children with wheezing

作　　者：季伟[1] 孙激扬[1] 陈正荣[1] 周卫芳[1] 严永东[1] 

机构地区：[1]苏州大学附属儿童医院呼吸科,215003

出　　处：《江苏医药》2010年第13期1514-1516,共3页Jiangsu Medical Journal

基　　金：苏州社会发展科技计划项目(SS0702)

摘　　要：目的探讨血小板活化及其相关炎症因子在伴有喘息症状儿童肺炎中的作用。方法住院33例肺炎患儿分为喘息症状(A组,21例)和无喘息症状(B组,12例)两组;对照(C,10例)组为健康儿童。采用流式细胞术检测外周血血小板膜CD62P+,ELISA检测血浆P-选择素以及转化生长因子β(TGF-β)的水平。结果 A、B组血小板膜CD62P表达均高于C组(P<0.05)。胶原刺激前三组P-选择素和TGF-β差异无统计学意义;胶原刺激后三组P-选择素和TGF-β均增高,A、B组显著增高于C组,且A组增高幅度显著高于B组。结论对喘息患儿进行早期干预血小板活化治疗可望成为预防哮喘的一种新途径。Objective To investigate the role of platelet activation and platelet-related inflammatory factor in pneumonia children with wheezing.Methods The pneumonia children diagnosed at admission were divided into group A with wheezing in 21 cases and group B without wheezing in 12 cases.Ten healthy children（group C） were taken as the controls.The expression of CD62P on platelets was determined by flow cytometery and plasma transforming growth factor-β（TGF-β） and P-selectin levels were detected by ELISA.Results The expression of CD62P was significantly higher in groups of A and B than that in group C（P0.05）.There was no significantly difference in the expressions of TGF-β and P-selectin before collagen stimulatation among three groups.After the platelet-rich plasma stimulated with collagen for 30 minutes,plasma levels of TGF-β and P-selectin were significantly higher than those before in all three groups,which were higher in groups of A and B than those in group C and higher in group A than those in group B.ConclusionThe treatment against platelet activation chould be taken in the future as a preventive way in chidren with wheezing.

关 键 词：肺炎 喘息 CD62P 转化生长因子β P-选择素 

分 类 号：R725[医药卫生—儿科]