NOD小鼠视网膜VEGF表达和视网膜细胞凋亡(英文)  

VEGF expression and cell apoptosis in NOD mouse retina

作　　者：李才锐[1] 孙曙光[1] 

机构地区：[1]云南大理学院附属医院眼科,中国云南省大理市671000

出　　处：《国际眼科杂志》2010年第8期1459-1462,共4页International Eye Science

摘　　要：目的:研究NOD小鼠早期糖尿病视网膜VEGF表达和视网膜细胞凋亡情况,以及二者间的关系。方法:NOD小鼠分为对照组(非糖尿病小鼠)(2,4,6,8,12wk组,n=30)和糖尿病组(2,4,6,8,12wk组,n=30)。每组小鼠在规定时间处死,提取血液标本,摘除眼球,分离视网膜备用。ELISA法检测视网膜VEGF和血液VEGF。透射电子显微镜检测小鼠视网膜细胞凋亡情况。结果:糖尿病组血液和视网膜VEGF表达与对照组相比明显增高(12wk,血液标本:4.9±0.4μg/gvs0.19±0.1μg/g,P<0.01;视网膜,165.0±9.0μg/gvs18.0±4.0μg/g,P<0.01)。NOD小鼠早期糖尿病视网膜VEGF表达和血液VEGF表达呈正相关(γ=0.9902,P=0.001)。糖尿病组视网膜神经节细胞和血管内皮细胞凋亡明显增加P<0.01。结论:视网膜VEGF表达增加可能与视网膜凋亡增多有关。早期糖尿病NOD小鼠视网膜VEGF表达增加是多因素的。·AIM：To investigate retinal vascular endothelial growth factor（VEGF）level and retinal cells apoptosis in the early stage of diabetic NOD mouse retina.·METHODS：Animals were divided into the control group（non-diabetes mice）（2,4,6,8,12 weeks group,n=30）and diabetes group（2,4,6,8,12 weeks group,n=30）.ELISA（Enzyme-Linked Immunosorbent Assay）was performed to detect VEGF level in both serum and retina.Transmission electron microscope method was used to examine retinal cell apoptosis.·RESULTS：Compared with the control group,VEGF levels in serum and retina were increased significantly in the NOD group（12 weeks：4.9±0.4μg/g versus 0.19±0.1μg/g in serum sample,P0.01;165.0±9.0μg/g versus 18.0±4.0μg/g in retinal sample,P0.01）.There exists a positive correlation between serum VEGF and retinal VEGF levels in the early diabetic NOD mice（γ=0.9902,P=0.001）.The number of the cells apoptosis in the ganglion cells and endothelium can also been found increased significantly in the NOD group（P0.01）.·CONCLUSION：The high VEGF expression may be contributed to increase retinal cells apoptosis.Many factors associated with retinal VEGF expression might involve in the early diabetes stage.·

关 键 词：视网膜 凋亡 血管内皮细胞 NOD小鼠 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]