原花青素对紫外线诱导晶状体上皮细胞氧化损伤保护作用的研究  

Study on protection of procyanidins against UV-induced oxidative damage of lens epithelial cells

作　　者：于攀[1] 王欣玲[1] 阎启昌[1] 

机构地区：[1]中国医科大学附属第四医院眼科,中国医科大学眼科医院,辽宁省晶状体重点实验室,中国辽宁省沈阳市110005

出　　处：《国际眼科杂志》2010年第8期1477-1480,共4页International Eye Science

基　　金：中国辽宁省自然科学基金资助项目(No.20092101);中国辽宁省教育厅重点实验室基金资助项目(No.2009S111,LS2010177)~~

摘　　要：目的:研究原花青素(procyanidins,PC)对紫外线诱导的人晶状体上皮细胞(len epithelial cells,LECs)DNA氧化损伤的保护作用。方法:采用照射剂量为15mJ/cm2的中波紫外线(UVB)分别照射用0(对照组),0.05,0.1,0.2mg/mL的PC,牛磺酸(TAU),维生素C(VC)预处理的LECs,未处理组未给予紫外线和任何抗氧化剂处理,用单细胞凝胶电泳法(SCGE)检测分析各PC组LECs DNA单链断裂的程度,并与其他各组的检测结果相比较。结果:各PC组的尾长、尾部DNA百分比、尾力矩在数值上均明显小于对照组,且与对照组相比,差异均有统计学意义(P<0.01)。各PC实验组的尾部DNA百分比、尾力矩与未处理组相比差异均无统计学意义(P>0.05)。相同浓度下,PC,TAU和VC组的各检测指标数值逐渐增大。结论:外源性PC对UVB照射诱导损伤的人LECs DNA有保护作用。PC的保护作用明显强于TAU和VC,并且在一定浓度范围内随着浓度的增加其抗氧化作用逐渐增强。·AIM：To determine the protection of procyanidins（PC）against UVB-induced oxidative damage of lens epithelial cells（LECs）with alkaline single cell gel electrophoresis,in order to provide an experimental foundation for cataract clinical treatment.·METHODS：LECs lines of generation 4 were divided randomly into 5 groups.As untreated group：normal cultur-ed LECs;control group：LECs ＋ UVB;three treated groups：PC treated groups：LECs ＋ PC ＋ UVB;taurine（TAU）treat-ed groups：LECs ＋ TAU ＋ UVB;VC treated groups：LECs＋VC＋UVB.All groups of antioxidants were divided into 3 groups according to the final concentration from low to high（0.05,0.1,0.2mg/mL）.Each treated group was treated by antioxidants 2 hours before irradiated under UVB,respectively,then conventional cultivation.The LECs of treated groups and control group were all irradiated at UVB-dose 15mJ/cm2.All of LECs in every group were collected after treatment an hour respectively and analyzed with alkaline single cell gel electrophoresis.·RESULTS：The differences of tail length,percentage of tail DNA and tail moment between PC treated groups and control group were statistically significant（P〈0.01）.The differences of percentage of tail DNA,tail moment in each PC treated group and untreated group were not significant（P〉0.05）.The results of PC treated groups were signi-ficantly smaller than those of TAU treated groups and VC treated groups,The differences of tail length,percent-age of tail DNA and tail moment in various antioxidant treated groups were statistically significant（P〈0.01）.·CONCULSION：Exogenetic PC has protective effect to UVB-induced LECs DNA damage.The protective effect of PC is obviously stronger than those of TAU and VC,and in a certain range of concentration,the protective effect of PC has the positive relationship of dose-effect.·

关 键 词：原花青素 紫外线 晶状体上皮细胞 DNA损伤 

分 类 号：R285[医药卫生—中药学]