土牛膝的急性毒性实验  

Experimental studies on acute toxicity of Achyranthes Aspera

作　　者：马文杰[1] 黄志芳[3] 黄志红[2] 胡吉林[2] 吴甜[2] 魏得良[2] 

机构地区：[1]湖南省湘南学院附一医院、郴州市第一人民医院综合科,423000 [2]湖南省湘南学院化学与生命科学系 [3]广州市第十二人民医院急诊科

出　　处：《国际中医中药杂志》2010年第4期349-350,共2页International Journal of Traditional Chinese Medicine

基　　金：基金项目：湖南省教育厅科研基金资助项目（项目编号：05C630）,湖南省科技厅科研基金资助项目（项目编号：06FJ4099）

摘　　要：目的 观察土牛膝的急性毒性反应,了解其对主要脏器的损伤情况.方法 本实验分两步,预试验初步确定用药剂量,正式实验采用40只小鼠,随机分为空白对照组和4个剂量组分别为400g/kg、300g/kg、225 g/kg、169 g/kg,连续灌胃7 d,观察小鼠症状表现及死亡情况,通过统计学处理得出半数致死量（LD50）.并对死亡小鼠进行解剖,观察器官的损伤情况.结果 小鼠的LD50为309.21 g/kg、标准差（Sx）=0.0359 g/kg,LD50的95%可信限为309.14～309.28 g/kg,按毒性分级标准,土牛膝为无毒类物.病理切片显示,大剂量给药后小鼠肝、脾组织均有一定程度的淤血.结论 土牛膝毒性较小,大剂量给药对小鼠心脏有一定影响.Objective To observe the acute toxicity of local achyranthes aspera, and its injury to major internal organs. Methods Estimated the dose before the experiment, then selected 40 mice and divided them into five groups randomly, namely 4 dose-groups and one control group. The doses used in the dose-groups were 400 g/kg, 300 g/kg, 225 g/kg and 169 g/kg respectively. Observed the symptoms and the death for successive 7 days and calculated LD （50）with statistic methods. Dissected the dead mice and observed the lesion of organs. Results The LD50 of acute toxicity test of achyranthes aspera was 309.21 g/kg, Sx=0.0359 g/kg. 95% of the limit of trust was 309.14～309.28 g/kg. According to the acute toxic standard, it belonged to non-toxicity. The specimen revealed that large dose administration caused coagulation of blood in both liver and spleen. Conclusion Local achyranthes aspera had little toxicity. Large dose administration affected heart function of mice.

关 键 词：土牛膝 小鼠 急性毒性 

分 类 号：R285.5[医药卫生—中药学]