鹅血液基因组DNA的简单快速提取方法研究  

Simple and rapid extraction method of goose blood genome DNA

作　　者：吴慧英[1] 刘铀[2] 贾汝敏[1] 刘艳芬[1] 张丽[1] 

机构地区：[1]广东海洋大学动物科学系,广东湛江524088 [2]广东海洋大学动物医学系,广东湛江524088

出　　处：《广东畜牧兽医科技》2010年第4期29-31,共3页Guangdong Journal of Animal and Veterinary Science

基　　金：湛江市科技招标项目(0609135);广东海洋大学基金项目(C04095);校企合作项目(0810314)

摘　　要：本研究针对家禽血液红细胞有细胞核的特点,研究适合鹅血液基因组DNA的廉价、简便、快速的DNA提取方法,并为其它禽(鸟)类相关操作提供参考。通过裂解细胞,利用简便快速的操作方法将蛋白质和核酸分离,去除杂质,沉淀核酸,获得大量基因组DNA,并通过基因扩增检测其质量和效果。该方法提取的基因组DNA电泳检测条带明量,无拖尾,含量及纯度均较高,能够用于分子生物学实验研究。与传统的酚-氯仿DNA抽提方法相比,本实验建立的血液基因组DNA提取方法具有成本低、操作步骤简便和快速的特点,特别是对大量样品的提取更为实用。Simple and rapid DNA extraction method was established according to the feature that the avian red blood cells have nucleolus, which would provide references for other fowls （birds）. Blood cells were lysed and then the nucleic acid was separated from proteins. Then the genome DNA was acquired after precipitation. The quality of obtained DNA was detected by PCR and electrophoresis. The obtained genome DNA was complete and highly pure. Thus it could be used as the templete for further analysis. The results showed that this DNA extraction method was simple, fast and with low cost. It was especially adapt to the extraction of large-scale samples.

关 键 词：DNA提取方法 鹅血液 

分 类 号：S814.8[农业科学—畜牧学]