利用不同作图软件构建响叶杨×银白杨遗传图谱  

Comparison of Genetic Linkage Maps of Populus adenopoda × P. alba Hybrid Constructed by Different Mapping Softwares

作　　者：王源秀[1,2] 徐立安[2] 黄敏仁[2] 王明庥[2] 张美荣[1] 

机构地区：[1]安徽师范大学生命科学学院重要生物资源保护与利用研究安徽省重点实验室,芜湖241000 [2]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京210037

出　　处：《林业科学》2010年第7期55-63,共9页Scientia Silvae Sinicae

基　　金：林木遗传与生物技术省部共建教育部重点实验室开放课题(FGB200802);安徽师范大学博士启动基金;安徽师范大学生命科学学院重点实验室开放基金

摘　　要：利用FsLinkageMap2.0和JoinMap4.0软件构建响叶杨×银白杨F1遗传整合图谱,在重组率为0.245时,二者构建的遗传框架图都包含20个主要的连锁群(4个以上标记),但2个遗传框架图的图距大小差异显著,利用JoinMap4.0构建的遗传图谱的标记排列顺序更加符合杨树一致性图谱上的标记排列顺序。估计杨树基因组总长度为2695.56cM,图谱的基因组覆盖度为93.34%。将利用MapMaker3.0和FsLinkageMap2.0构建的母本响叶杨的2个遗传图谱进行对比,共检测到32个对应连锁群和113个相同标记,利用FsLinkageMap2.0构建的响叶杨的遗传图谱上共显性标记的排列顺序与标记在杨树全基因组中的相对位置更符合。Two genetic integrated maps of Populus adenopoda × P. alba were constructed using FsLinkageMap 2. 0 and JoinMap 4. 0 softwares in this study. On recombination ratio = 0. 245,two frame maps were all composed of 20 groups （containing more than 4 markers）. There was significant difference in the map distances between two frame maps and the codominant marker order of the map constructed by JoinMap4. 0 was more congruous with the Populus consensus map than that by FsLinkageMap2. 0. The estimated genome length was 2 695. 56 cM and covered 93. 34% of the total length of the genome of Populus adenopoda × P. alba. Two maternal maps constructed with FsLinkageMap 2. 0 and MapMaker 3. 0 had thirty-two pairs of corresponding linkage groups which were built up with 113 common markers. The codominant marker order of the map constructed by FsLinkageMap2. 0 was more coincident with the relative marker position in the poplar whole genome than that by MapMaker 3. 0.

关 键 词：响叶杨 银白杨 遗传图谱 SSR SRAP 

分 类 号：S718.46[农业科学—林学]