内皮素-1和血管内皮生长因子及缺氧诱导因子-1在门脉高压性胃病中的作用  

Endothelin-1,vascular endothelial growth factor and hypoxia inducible factor-1 in patients with portal hypertensive gastropathy

作　　者：李树娟[1] 邵石祥[1] 耿国钰[1] 蔡莉芬[1] 卢建华[1] 何朝霞[1] 叶立红[1] 

机构地区：[1]河北省石家庄市第五医院,050021

出　　处：《河北医药》2010年第15期1989-1991,共3页Hebei Medical Journal

基　　金：河北省石家庄市科技支撑计划项目(编号:071461033)

摘　　要：目的探讨患者胃黏膜是否有内皮素-1(ET-1)、血管内皮生长因子(VEGF)和缺氧诱导因子-1(HIF-1)在门脉高压性胃病(PHG)血流动力学调节中的意义。方法分别采取PHG、PHT无PHG、正常对照患者胃黏膜标本用免疫组织化学方法分别检测ET-1、VEGF和HIF-1在胃黏膜中的表达。同时测定3组患者的门静脉血流量(portal venous flow,PVF)和脾静脉血流量(spleen venous flow,SVF)。结果 PHG积分与食管静脉曲张程度无相关性(HC=4.782,P>0.05)。PHG组PVF和SVF水平明显高于正常对照组(P<0.05)。PHG组胃黏膜中ET-1、VEGF、HIF-1的阳性面积高于正常对照组(P<0.05)。ET-1、VEGF、HIF-与PHG积分呈正相关(H分别为10.592、10.614、9.852,P<0.05)。PHG组患者HIF-1阳性表达和VEGF的阳性表达之呈直线相关(Pearson相关数为0.842,P<0.01)。结论 ET-1、VEGF和HIF-1与PHG的发生发展有相关性。Objective To investigate the significance of endothelin-1（ ET-1） ,vascular endothelial growth factor （ VEGF） and hypoxia-inducible factor -1 （ HIF-1） in patients with portal hypertensive gastropathy（ PHG）. Methods The specimens of gastric mucosa were divided into three groups,patients with PHG group,PHT without PHG group and control group. The specimens were detected by immunohistochemical methods for the levels of ET-1,VEGF and HIF-1 respectvely. At the same time,the portal vein blood flow（ PVF） and splenic blood flow （ SVF） were detected in the three groups. Results PHG scoring was not related with the degree of esophageal varices（ P 〉0. 05）. The levels of PVF and SVF in PHG group were significantly higher than those of control group（ P 〈0. 05）. The positive area of ET-1,VEGF and HIF-1in PHG group was significantly higher than that of control group （ P 〈0. 05）. The levels of ET-1,VEGF,HIF-1 were positively correlated with PHG scoring（ P〈 0. 05）. The positive expression of HIF-1in PHG group showed a straight line correlation with the positive expression of VEGF （ Pearson correlation coefficient：0. 842,P 0. 01）. Conclusion The levels of ET-1,VEGF and HIF-1 are correlated with the pathogenesis and development of PHG.

关 键 词：内皮素-1 血管内皮生长因子 缺氧诱导因子-1 门静脉高压性胃病 免疫组化 

分 类 号：R657.34[医药卫生—外科学] R573[医药卫生—临床医学]