ε-己内酯/L-丙交酯聚合物组织工程血管支架材料的生物相容性研究  

EXPERIMENTAL STUDY ON BIOCOMPATIBILITY OF VASCULAR TISSUE ENGINEERING SCAFFOLD OF ε-CAPROLACTONE AND L-LACTIDE

作　　者：李春民[1] 董建德[2] 谷涌泉[3] 叶霖[4] 陈晓波[1] 卞策[1] 冯增国[4] 汪忠镐[3] 

机构地区：[1]首都医科大学附属复兴医院血管外科,北京100038 [2]北京电力总医院胸心血管外科 [3]首都医科大学宣武医院血管外科 [4]北京理工大学材料科学与工程学院

出　　处：《中国修复重建外科杂志》2010年第8期988-992,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家高技术研究发展计划(863)资助项目(2006AA02A134);国家自然科学基金资助项目(30640078)~~

摘　　要：目的探讨ε-己内酯/L-丙交酯聚合物(ε-caprolactone and L-lactide,PCLA)可降解组织工程血管支架材料的生物相容性,为进一步构建组织工程血管提供依据。方法取PCLA材料行大体和扫描电镜观察。行生物相容性检测,包括细胞相容性实验:将L929细胞接种于96孔培养板内,实验组加入PCLA材料浸提液,对照组加入DMEM完全培养基,MTT法7d内每天测定各组吸光度(A)值;急性全身毒性实验:分别将PCLA浸提液及等量生理盐水注入实验组和对照组小鼠腹腔内(n=5),72h内观察小鼠毒性表现;溶血实验:取抗凝兔血加入浸提液、生理盐水、三蒸水,测定各组A值;细胞贴附实验:L929细胞接种于PCLA表面,培养4h及3d后扫描电镜观察;皮下植入实验:将PCLA材料植入大鼠皮下,1、8周后行组织学观察。结果支架外观呈乳白色,质地均匀,具有良好柔韧性及弹性。扫描电镜观察可见超细纤维粗细均匀、外表平滑;纤维直径1～5μm;孔隙直径10～30μm。细胞相容性实验示实验组与对照组细胞生长曲线类似(P>0.05)。急性全身毒性实验示两组小鼠在观察期内活动及进食情况均良好,无呼吸抑制现象,无瘫痪、惊厥及死亡发生。溶血实验:溶血率为1.18%,低于国家标准(GB-T14233.2-2005)规定的5%。细胞贴附实验示细胞在材料表面能够充分贴附生长。皮下植入实验示材料与组织相容性好。结论 PCLA材料具有良好的显微结构,安全无毒性,具有良好的生物相容性,是一种理想的组织工程血管支架材料。Objective To explore the method of preparing the electrospinning of synthesized triblock copolymers of ε-caprolactone and L-lactide （PCLA） for the biodegradable vascular tissue engineering scaffold and to investigate its biocompatibility in vitro. Methods The biodegradable vascular tissue engineering scaffold was made by the electrospinning process of PCLA. A series of biocompatibility tests were performed. Cytotoxicity test： the L929 cells were cultured in 96-well flat-bottomed plates with extraction media of PCLA in the experimental group and with the complete DMEM in control group, and MTT method was used to detect absorbance （A） value （570 nm） every day after culture. Acute general toxicity test： the extraction media and saline were injected into the mice’s abdominal cavity of experimental and control groups, respectively, and the toxicity effects on the mice were observed within 72 hours. Hemolysis test： anticoagulated blood of rabbit was added into the extracting solution, saline, and distilled water in 3 groups, and MTT method was used to detect A value in 3 groups. Cell attachment test： the L929 cells were seeded on the PCLA material and scanning electron microscope （SEM） observation was performed 4 hours and 3 days after culture. Subcutaneous implantation test： the PCLA material was implanted subcutaneously in rats and the histology observation was performed at 1 and 8 weeks. Results Scaffolds had the characteristics of white color, uniform texture, good elasticity, and tenacity. The SEM showed that the PCLA ultrafine fibers had a smooth surface and proper porosity; the fiber diameter was 1-5 μm and the pore diameter was in the range of 10-30 μm. MTT detection suggested that there was no significant difference in A value among 3 groups every day after culturing （P 0.05）. The mice in 2 groups were in good physical condition and had no respiratory depression, paralysis, convulsion, and death. The hemolysis rate was 1.18% and was lower than the normal level （5%）. The S

关 键 词：血管组织工程 ε-己内酯/L-丙交酯聚合物 支架材料 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程] Q813[医药卫生—基础医学]