系统性红斑狼疮患者外周血淋巴细胞bcl-2的表达及临床意义  

Expression of bcl-2 in Lymphocytes from Patients with Systemic Lupus Erythematosus and Its Clinical Significance

作　　者：刘亚光[1] 尹培达[1] 石成钢[1] 赵湘茹[2] 孔庆瑜[1] 黄必军[2] 

机构地区：[1]中山医科大学附属第一医院风湿科,广州510080 [2]中山医科大学附属第一医院,广州510080

出　　处：《中华皮肤科杂志》1999年第1期9-11,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助!(39770706)

摘　　要：目的为了探讨bcl 2基因在系统性红斑狼疮(SLE)发病机制中的作用及临床意义。方法应用逆转录 -聚合酶链反应(RT PCR)检测31例SLE患者外周血单一核细胞(PBMC)bcl 2mRNA表达水平和流式细胞仪双标记法分析其T、B细胞bcl 2蛋白表达。结果活动期SLE患者PBMCbcl 2mRNA表达水平明显升高 ,占55 6 % ,且活动期SLE患者CD3+ 、CD4+ 和CD8+ T细胞亚群bcl 2蛋白表达明显高于非活动期SLE患者、正常人对照组和其他疾病组 ,而CD19+ B细胞bcl 2蛋白表达在各组之间并无统计学差异。结论T细胞bcl 2异常表达在SLE疾病活动的过程中起着重要作用 ,检测SLE患者外周血T淋巴细胞bclObjective To investigate the role of bcl 2 expression in the pathogenesis of systemic lupus erythematosus (SLE) and its clinical significance Methods The bcl 2 mRNA levels in peripheral blood mononuclear cells (PBMC) from 31 SLE patients were tested by reverse transcription polymerase chain reaction (RT PCR), and the bcl 2 protein levels in T and B cells were also tested by two colour cytofluorography Results bcl 2 mRNA levels in PBMC increased significantly in 10 cases of active SLE, accounting for 55 6% of active SLE patients Compared with those in inactive SLE patients, normal controls and other non SLE patients, the bcl 2 protein expression in T cell subsets (including CD3+, CD4+ and CD8+ ) increased significantly in active SLE patients However, there was no statistical difference in bcl 2 protein levels in CD19+ B cells among all groups Conclusion The abnormal expression of apoptosis related bcl 2 in T cells might play an important role in disease activity of SLE , and the determination of bcl 2 expression levels of peripheral T lymphocytes might be used as an index for disease activity of SLE

关 键 词：红斑狼疮 系统性 BCL-2基因 细胞凋亡 

分 类 号：R593.241[医药卫生—内科学]