视黄酸受体和类视黄醇X受体激动剂对脐血单个核细胞IL-4和IFN-γ表达的影响  

Effects of retinoic acid receptor agonist and retinoid X receptor agonist on the expression of IL-4 and IFN-γ in cord blood mononuclear cells

作　　者：朱东洋[1] 王黎荔[1] 李云[1] 朱彦锋[1] 赵品楠[1] 

机构地区：[1]四川大学华西公共卫生学院营养与食品卫生学教研室,成都610041

出　　处：《卫生研究》2010年第4期433-435,共3页Journal of Hygiene Research

基　　金：国家自然科学基金(No.30671761)

摘　　要：目的观察视黄酸受体(RARs)激动剂四氢四甲基萘丙烯苯甲酸乙酯(TTNPB)和类视黄醇X受体(RXRs)激动剂甲氧普烯酸(MA)对脐血单个核细胞表达IL-4和IFN-γ的影响。方法采用ELISA和RT-PCR,分别从蛋白质和基因水平上观察TTNPB和MA对脐血单个核细胞表达IL-4和IFN-γ的影响。结果 ELISA显示10-8、10-6、10-5、10-4mol/L的TTNPB和10-8、10-5、10-4mol/L的MA显著降低IFN-γ/IL-4蛋白含量比值(P<0.05);RT-PCR显示10-4mol/L的MA显著降低IFN-γ/IL-4mRNA比值(P<0.05),而10-5mol/L的TTNPB和10-7、10-6mol/L的MA显著升高IFN-γ/IL-4mRNA比值(P<0.05)。结论适当浓度的TTNPB和MA可以在蛋白质水平上下调IFN-γ/IL-4的比值;不同浓度的TTNPB和MA在基因水平上对IFN-γ/IL-4的比值有不同影响。Objective To observe the effects of retinoic acid receptors agonist TTNPB and retinoid X receptors agonist MA on both protein and gene expression of IFN-γ and IL-4 in cultured cord blood mononuclear cells.Methods Observing both the protein content and gene expression of IFN-γ and IL-4 with ELISA and fluorescent real-time quantitative PCR in vitro.Results It was indicated by ELISA that 10^-8 ,10^-6 ,10^-5 and 10^-4 mol /L TTNPB and 10^-8 ,10^-5 and 10^-4 mol /L MA decreased the ratio of IFN-γ /IL-4 significantly in comparison with the control （P〈0.05 ）,and indicated by RT-PCR that 10^-4 mol /L MA decreased the ratio of IFN-γ /IL-4mRNA significantly and 10^-5 mol /L TTNPB and 10^-7 , 10^-6 mol /L MA increased the ratio of IFN-γ /IL-4mRNA significantly in comparison with the control（ P〈0.05）.Conclusion The ratio of IFN-γ /IL-4 could be down - regulated by proper concentration of TTNPB and MA at the protein level,while the ratio of IFN-γ /IL-4mRNA was differently influenced by various concentration of TTNPB and MA at the gene level.

关 键 词：视黄酸受体激动剂 类视黄醇X受体激动剂 单个核细胞 白介素4 干扰素Γ 维生素A 

分 类 号：Q562[生物学—生物化学] Q26