马铃薯X病毒黑龙江分离物外壳蛋白基因克隆与序列分析  

Cloning and Sequences Analysis of CP Gene of Potato Virus X Heilongjiang Isolate

作　　者：张威[1] 白艳菊[1] 申宇[1] 高艳玲[1] 范国权[1] 耿宏伟[1] 王魏[1] 孟宪欣[2] 

机构地区：[1]黑龙江省农业科学院植物脱毒苗木研究所,黑龙江哈尔滨150086 [2]黑龙江省农业科学院作物育种研究所,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2010年第8期1-5,共5页Heilongjiang Agricultural Sciences

基　　金：948资助项目(2008-Z23);马铃薯产业技术体系资助项目(NYCYTX-15);农业科技成果转化资助项目(2008GB2B200084)

摘　　要：马铃薯X病毒（Potatovirus X，PVX）是重要的马铃薯病毒之一，几乎分布于全国所有马铃薯种植区域，严重影响马铃薯的产量和品质。对其建立RT—PCR分子检测体系以及对其外壳蛋白（CP）基因进行序列分析尤为重要。根据已报道的PVX的核苷酸序列设计合成1对引物，以带毒植株总RNA为模板，应用RT—PCR方法，克隆了PVX黑龙江分离物（P2）全长CP基因序列。序列分析表明：P2CP基因全长711bp，编码237个氨基酸残基，与GenBank中17个不同分离物的CP核苷酸序列同源性为75．5％～96．3％，氨基酸序列同源性为89．4％～99．2％。依据PVX CP氨基酸序列建立系统进化树，将PVX不同分离物划分为两大类群，P2与PVX中国分离物亲缘关系较近，同属于类型Ⅱ，表现一定的地域相关性。同时，应用该RT—PCR分子检测体系进行马铃薯样品检测。Potato virus X（PVX）is the impotaint virus disease in potato,distributing nearly in all the potato planted area,can affect the yield and quality of potato seriously, so erecting RT-PCR molecule detecting system and CP sequence analysising are important. A pair of primers was designed based on the reported CP gene of PVX the hole CP gene sequence of PVX Heilongjiang isolate was cloned by reverse transcription polymerase chain reaetion（RT-PCR）. The analysis of sequence showed that： The CP gene of P2 was made up of 711 nucleic acids,coding 237 amino acids. The CP nucleic acid sequence homology of P2 and other 17 isolates of PVX in GenBank was between 75.5%-96.3% ,the CP amino acid sequence homology was between 89.4%- 99.2%. The phylogenic tree which established based on PVX CP amino acid was clustered into two groups, P2 has nearer sibship with the isolates of China, both in Ⅱ group, they represented region correlation. Finally, using the RT-PCR detection system to detect potato virus.

关 键 词：马铃薯X病毒 外壳蛋白 核苷酸 序列分析 亲缘关系 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]