TAIL-PCR方法研究花粉管通道法转化机理初探  

Study on the mechanism of pollen-tube pathway-mediated transformation method with TAIL-PCR

作　　者：郭金英[1] 张天真[2] 

机构地区：[1]河北工程大学,河北邯郸056038 [2]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《河北农业大学学报》2010年第4期5-9,共5页Journal of Hebei Agricultural University

基　　金：长江学者和创新团队发展计划资助项目(IRT0432)

摘　　要：本研究以通过花粉管通道法获得的Bt+Sck双价基因棉转基因植株为材料,利用改进的TAIL-PCR方法扩增T-DNA插入位点左、右边界的侧翼序列,结果表明:T-DNA侧翼序列均包含一段载体骨架序列;T-DNA插入区富含A,T碱基对,并且属于核基质结合区(Bind to nuclear matrices);T-DNA整合到棉花基因组后,其左、右边界均有缺失。花粉管通道转化的机理很复杂,该试验为进一步研究此转化方法提供了一种实用的研究方法。TAIL-PCR was used to obtain the flanking sequence of T-DNA insertion in transgenic cotton with Bt＋Sck developed by pollen-tube pathway-mediated transformation.The results showed that the flanking sequences contained a section of vector sequence and were rich in ＇A＋T＇ base pairs bound to nuclear matrices.Furthermore,the left or right border of T-DNA was absent during the T-DNA insertion process.Although the mechanism of pollen-tube pathway-mediated transformation method is very complex,this study will provide practical help for study on the mechanism of this transformation method.

关 键 词：TAIL-PCR 花粉管通道转化方法 棉花 

分 类 号：S565.1[农业科学—作物学]