ROCK选择性抑制剂Y-27632提高小鼠胚胎干细胞传代效率研究  

Selective inhibitor of the ROCK (Rho-associated kinase inhibitor) Y-27632 enhances passage effect of mouse embryonic stem cells

作　　者：张鹏[1] 赵虎[1] 吴兴龙[1] 李相运[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001

出　　处：《河北农业大学学报》2010年第4期94-98,共5页Journal of Hebei Agricultural University

基　　金：国家自然科学基金资助项目(30871790);河北省自然科学基金项目(C2009000600)

摘　　要：以小鼠胚胎干细胞(ES细胞)为研究对象,探讨ROCK选择性抑制剂Y-27632在细胞传代过程中的作用,并对ES细胞相关生物学特性进行检测。结果表明:Y-27632能够引起ES细胞形态发生改变,通过Y-27632的添加能够使质地紧密的ES细胞集落变得松散、扁平。将Y-27632应用于ES细胞传代,能够显著提高传代效率。并且,在Y-27632长期存在的条件下,ES细胞依旧维持稳定的染色体数目,较强的碱性磷酸酶活性,在体内能够分化形成畸胎瘤,在体外能够分化形成心肌细胞。说明Y-27632的使用能够显著提高小鼠ES细胞的传代效率;紧密的细胞连接不是维持ES细胞多能性的必要条件。This study took mouse embryonic stem cells（ES cells） as the research object,aiming to evaluate the effect of selective inhibitor of the ROCK（Rho-associated kinase inhibitor） Y-27632 on passage,and examine the biological characteristics of ES cells via Y-27632 treatment.The results indicated that Y-27632 could change the morphology of ES colonies.Y-27632 could turn tight cell-cell connection into loose and flat connection.In passage,Y-27632 could improve the efficiency.Furthermore,under the condition of long-standing Y-27632,ES cells remained the stable number of chromosome,high alkaline phosphatase activity,and differentiated into teratomas in vivo,cardiomyocytes in vitro.These observations suggest that Y-27632 can significantly improve efficiency of passage on ES cells.Tight cell-cell connection is not the indispensable condition for pluripotency of ES cells.

关 键 词：小鼠胚胎干细胞 ROCK抑制剂 Y-27632 

分 类 号：Q813[生物学—生物工程]