氯化镉致人肝癌细胞SMMC-7721线粒体损伤作用  

Mitochondrial damage in SMMC-7721 cells induced by cadmium chloride

作　　者：刘晓梅[1] 石磊[1] 王雯[1] 金明华[1] 杜海英[1] 刘颖[1] 孙磊[1] 孙志伟[1,2] 

机构地区：[1]吉林大学公共卫生学院,吉林长春130021 [2]首都医科大学公共卫生与家庭医学学院,北京100069

出　　处：《癌变．畸变．突变》2010年第4期276-278,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然基金青年基金(30700672)

摘　　要：目的:研究氯化镉对人肝癌细胞SMMC_7721线粒体的损伤作用。方法:分别用5、10、20、30、40μmol/L氯化镉作用体外培养的SMMC_7721细胞24h,实验同时设对照组。采用形态学方法检测其线粒体的结构完整性,用分光光度法检测线粒体钙离子转运能力,采用琼脂糖凝胶电泳法检测线粒体DNA损伤。结果:随着氯化镉的浓度升高,存活细胞数降低,细胞内的线粒体数量减少;氯化镉处理组细胞线粒体钙离子转运能力下降,并可见线粒体DNA损伤条带。结论:氯化镉可以引起人肝癌细胞SMMC_7721线粒体及线粒体DNA损伤。OBJECTIVE：The mitochondrial damage of SMMC-7721 cells induced by cadmium chloride was studied in vitro.METHODS：The SMMC-7721 cells were treated with cadmium chloride for 24 hours.Morphology was used to assess integrity of mitochondria.Spectrophotography was used to measure the transport ability of Ca^2＋ into mitochondria and agarose gel electrophoresis was used to observe the mitochondrial DNA damage.RESULTS：The mumber of treated cell and mitochondria decreased with increasing cadmium concentration.The transport ability of Ca^2＋ into mitochondria was decreased and the mitochondrial DNA was damaged with increasing concentration.CONCLUSION：Cadmium chloride could damage the mitochondria and its DNA in SMMC-7721 cells.

关 键 词：氯化镉 SMMC-7721 线粒体 钙离子 

分 类 号：R994.6[医药卫生—毒理学]