Apg-2对H2O2诱导的BaF3-P210细胞损伤的影响  

Effect of Apg-2 on H_2O_2-induced damage to BaF3-P210 cells

作　　者：李春莉[1] 刘钉宾[1] 袁颖[2] 彭智[1] 陶崑[1] 史梦[1] 黄宗干[1] 冯文莉[1] 

机构地区：[1]重庆医科大学医学检验系临床血液学教研室,临床检验诊断学教育部重点实验室,重庆400016 [2]重庆医科大学附属第一医院普外科,重庆400016

出　　处：《第三军医大学学报》2010年第15期1621-1624,共4页Journal of Third Military Medical University

基　　金：教育部博士学科点专项基金(2005631007)~~

摘　　要：目的探讨Apg-2对H2O2诱导的Bcr/Abl阳性BaF3-P210细胞损伤的影响。方法以H2O2诱导的pMIGR1空载体感染细胞BaF3-MIGR1和稳定表达Bcr/Abl的BaF3-P210细胞为损伤模型,Western blot和RT-PCR检测H2O2损伤后2组细胞Apg-2表达;建立过表达Apg-2的BaF3-MIGR1细胞(BaF3-MIGR1-Apg2)和BaF3-P210细胞(BaF3-P210-Apg2)H2O2诱导的损伤模型,Western blot检测磷酸化组蛋白(γ-H2AX)的表达,免疫荧光法检测γ-H2AX焦点数。结果 H2O2作用后的BaF3-MIGR1和BaF3-P210细胞的Apg-2蛋白和mRNA水平表达均升高(P<0.05)。H2O2作用BaF3-MIGR1和BaF3-MIGR1-Apg2细胞后其γ-H2AX蛋白表达升高,γ-H2AX焦点数亦增加;BaF3-P210和BaF3-P210-Apg2细胞H2O2损伤后γ-H2AX蛋白表达和焦点数均无明显变化。结论 Apg-2可减少H2O2诱导的BaF3-P210细胞γ-H2AX表达,从而可能降低其细胞损伤。Objective To study the effect of Apg-2 on H2O2-induced damage to Bcr/Abl positive BaF3-P210 cells. Methods Damage models of BaF3-MIGR1 cells infected with pMIGR1 vector and BaF3- P210 cells stably expressing Ber/Abl were induced by H2O2. Expression of Apg-2 in the two models was detected by Western blotting and RT-PCR. Damage models of BaF3-MIGR1 and BaF3-P210 cells with over-expressed Apg-2 were also induced by H2O2. Expression of phosphorylated histone （γ-H2 AX） and its focal points was detected by Western blotting and immunofluorescence, respectively. Results The expression levels of Apg-2 protein and mRNA in BaF3-MIGR1 and BaF3-P210 cells were increased when Apg-2 was exposed to H2O2 （P 〈 0.05）. The expression levels of γ-H2AX and its focal points in BaF3-MIGR1 and BaF3-P210-Apg 2 cells were also increased after treatment with H2O2 （ P 〈 0.05 ） . However, no significant alternation was observed in expression of γ-H2AX in BaF3-P210 and BaF3-P210-Apg2 cells after treatment with H2O2. Conclusion Apg-2 can reduce H2O2 -induced expression of γ-H2AX in BaF3-P210 cells, thus decreasing cell damage.

关 键 词：Apg-2 H2O2慢性粒细胞白血病 Bcr/Ab1 细胞损伤 

分 类 号：R341[医药卫生—基础医学] R730.23