盐酸小檗碱诱导Jurkat细胞凋亡的研究  

Berberine-induced apoptosis in Jurkat cells

作　　者：赖广钦 林文盛 杨菁[2] 

机构地区：[1]福州神经精神病防治院,福州350008 [2]福建医科大学附属协和医院,福州350001

出　　处：《海峡药学》2010年第7期235-238,共4页Strait Pharmaceutical Journal

摘　　要：目的本研究旨在观察盐酸小檗碱能否抑制Jurkat细胞的增殖、并对其作用机制进行初步探讨,从而为临床上应用治疗急性T淋细胞白血病提供实验依据。方法 MTT比色法检测盐酸小檗碱对Jurkat细胞增殖的影响;AO/EB染色法观察细胞凋亡的形态学改变;TUNEL方法检测细胞凋亡的生化功能变化。结果盐酸小檗碱对Jurkat细胞的生长有抑制作用,呈现明显的剂量和时间依赖性,24h、48h、72h、96h、120h的IC_(50)分别为107.5567μg.mL^(-1)、23.5600μg.mL^(-1)、10.8069μg.mL^(-1)、9.2660μg.mL^(-1)和2.7121μg.mL^(-1),盐酸小檗碱处理Jurkat细胞可见典型的细胞凋亡形态学改变;TUNEL可见深棕色凋亡细胞。结论盐酸小檗碱能有效地抑制体外Jurkat细胞的增殖,其作用可能是通过诱导Ju-rkat细胞凋亡有关。OBJECTIVE Our research was carried out to investigate whether the inhibition of Berberine hydrochloride Jurkat cell proliferation and its mechanism of preliminary discussions. This study aims at supplying＇ the theoretical evidence for the clinical application of Coptis chinesis Franch for curing T acute lymphoblastic leukemia. METHODS The proliferation-inhibiting capability of Berberine was investigated by using MTT colorimetric assay; AO/EB staining to observe morphological changes of apoptosis; TUNEL detection of apoptosis changes in the biochemical function. RESULTS Berberine effectively inhibited the proliferation of Jurkat cells in a dose- and time-dependent way, the IC50 of 24 hours, 48 hours, 72 hours, 96 hours and 120 hours were 107. 5567μg·mL^-1 23. 5600μg·mL^-1, 10. 8069μg·mL^-1, 9. 2660μg·mL^-1and 2. 7121μg·mL^-1; When Berberihe treated Jurkat cells for 48 hours, it shows the typical morphological changes of apoptosis; The dark brown positive apoptotic cells were found by TUNEL. CONCLUSION Berberine effectively inhibited Jurkat cell proliferation in vitro, its role may be through induction of apoptosis of Jurkat cells.

关 键 词：小檗碱 JURKAT细胞 增殖 凋亡 

分 类 号：R963[医药卫生—微生物与生化药学]