胶原诱导性关节炎小鼠Th17细胞的检测及意义  

Determination and significance of Th17 cells in the mice of collagen-induced arthritis

作　　者：史烨[1] 马洁[1] 苏兆亮[1] 杨先知[1] 马斌[1] 田洁[1] 刘艳[1] 刘青玲[1] 邵启祥[1] 许化溪[1] 吕力为[2] 王胜军[1] 

机构地区：[1]江苏大学免疫学和免疫检验学系,江苏镇江212013 [2]香港大学病理学系

出　　处：《江苏大学学报（医学版）》2010年第4期299-301,共3页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30871193;30972748;30910103087);江苏省医学科研基金资助项目(200952);江苏省自然科学基金资助项目(08KJB320002);江苏大学"拔尖人才工程"资助项目

摘　　要：目的:研究Th17细胞及其相关细胞因子白细胞介素-17(IL-17)在胶原诱导性关节炎(collagen-induced ar-thritis,CIA)小鼠体内的变化。方法:利用Ⅱ型胶原蛋白建立小鼠CIA模型。应用流式细胞术(FCM)分析小鼠脾脏及引流淋巴结中Th17细胞的比例,采用酶联免疫吸附试验(ELISA)检测小鼠血清中IL-17的含量。结果:CIA小鼠脾脏及引流淋巴结中Th17细胞的比例分别为(3.19±0.70)%、(3.54±0.97)%,显著高于正常对照组的(0.95±0.34)%、(1.31±0.47)%,P<0.01。CIA小鼠血清中IL-17的含量也明显高于正常对照组(P<0.05)。结论:Th17细胞及其分泌的细胞因子IL-17在CIA小鼠体内明显升高,并与疾病的发生密切相关。Objective： To study the expression of Th17 cells and their cytokines （ IL-17 ） in the development. of collagen-induced arthritis （CIA） in mice. Methods： The CIA models were immunized with collagen type Ⅱ and Freund＇s adjuvant. The percentage of Th17 cells in spleens and draining lymph nodes were determined by flow-cytometry, and the concentration of IL-17 in the serum were examined by enzyme linked immunosorbent assay （ELISA） respectively. Results： Compared with the normal group, CIA mice had a significantly higher percentage of Th17 cells in spleens and draining lymph nodes （P 〈 0.01 ） and had an increased concentration of IL-17 in the serum （P 〈0.05）. Conclusion： There was a significantly elevated expression of Th17 cells and their cytokines （ IL-17 ） in the mice of CIA, which was related to the development of the disease

关 键 词：TH17细胞 白细胞介素-17 胶原诱导性关节炎 

分 类 号：R392.11[医药卫生—免疫学]