腺病毒载体介导EGFP转染大鼠骨髓间质干细胞研究  

A study on transfecting green fluorescent protein gene by adenovirus vector to rat bone marrow derived mesenchymal stem cells

作　　者：王兴忠 孟静 陈丽萍 吕汉琰 朱伟[2] 许文荣[2] 

机构地区：[1]无锡市亿仁医院检验科,江苏无锡214174 [2]江苏大学基础医学与医学技术学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2010年第4期310-314,共5页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:探讨腺病毒载体介导的绿色荧光蛋白基因转染大鼠骨髓间质干细胞(mesenchymal stem cells,MSCs)的生物学特性。方法:骨髓贴壁法分离培养大鼠骨髓MSCs并采用流式细胞术进行鉴定。利用脂质体法通过腺病毒转染获得EGFP修饰的大鼠骨髓MSCs。结果:成功分离得到大鼠MSCs,腺病毒转染后大鼠骨髓MSCs过表达绿色荧光蛋白(EGFP)报告基因,且转染后MSCs具有成骨分化潜能。结论:通过腺病毒转染能获得EGFP标记的大鼠MSCs,为MSCs组织工程和基因治疗研究提供了依据。Objective： To investigate the biological characteristics of rat bone marrow mesenchymal stem cells （MSCs） transfected green fluorescent protein（GFP） gene by adenovirus vector. Methods： Rat bone marrow-derived MSCs were isolated by adherent culture method. Flow cytometric analyses were used to identify cells which were transfected by recombinant adenovirus-EGFP using lipofectamine2000. Results： Rat bone marrow-derived MSCs were successfully isolated and efficiently express EGFP report gene by adenovirus vectors transfection. Conclusion： Rat bone marrow-derived MSCs can be transfected by recombinant adenovirus-EGFP, which may potentially be used as a target for tissue engineering and gene therapy.

关 键 词：间质干细胞 腺病毒载体 基因治疗 转染 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] Q78[医药卫生—基础医学]