谷胶蛋白对小鼠中枢神经系统发育和功能的影响  

Effects of gliadin on development and function of central nervous system in mice

作　　者：武宁[1,2] 王珍琦[1] 关松磊[1] 油洋[1] 张萱[1,3] 

机构地区：[1]吉林大学公共卫生学院基因组医学研究中心卫生部放射生物学重点实验室,吉林长春130021 [2]吉林大学中日联谊医院放射治疗科,吉林长春130033 [3]中国医学科学院基础所医学分子生物学国家重点实验室,北京100005

出　　处：《吉林大学学报（医学版）》2010年第4期607-610,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30700257);科技部医学分子生物学国家重点实验室外部课题(2009);吉林大学中日联谊医院青年科研基金资助课题(2009)

摘　　要：目的:探讨谷胶蛋白对小鼠中枢神经系统发育和功能的影响,阐明其可能是导致神经精神系统机能障碍的一种环境风险因子。方法:应用谷胶蛋白隔日灌胃刚离乳的BALB/c小鼠,辅以应激刺激,建立谷胶蛋白口服免疫模型。小鼠分为血清抗谷胶蛋白IgA阳性组和阴性组(每组10只),观察两组小鼠一般状态,应用悬挂实验、运动平衡及协调实验、旷场实验和强迫游泳实验检测小鼠行为学改变,计算脑指数。结果:与抗谷胶蛋白IgA阴性组比较,阳性组小鼠出现不同程度的活动减少、外表脏乱、反应迟钝及精神萎靡等状况;抗谷胶蛋白IgA阳性组小鼠体质量明显低于阴性组(P<0.05);与抗谷胶蛋白IgA阴性组比较,阳性组小鼠的运动平衡和协调保持时间及旷场运动得分明显降低,强迫游泳"不动状态"累积时间明显延长(P<0.05);抗谷胶蛋白IgA阳性组小鼠小脑质量与全脑质量的比值明显低于阴性组(P<0.05)。结论:针对谷胶蛋白的免疫反应可能影响幼年个体中枢神经系统的正常发育和功能。Objective To investigate the effects of gliadin on development and function of central nervous system in mice,and elucidate that gliadin is very likely to be an environmental factor for mental disorder.Methods Newly-weaning BALB/c mice were intragastrically administered with gliadin,and stimulated with stress.The mice were divided into positive anti-gliadin IgA aitibody group and negative anti-gliadin IgA antibody group（n=10）.The systemic-toxic effects of animal models induced by gliadin were detected with basic observation,ethological evaluation（Hang test,Balance and coordination test,Open-field test and Forced swimming test）and the cerebral index was calculated.Results The mice in positive anti-gliadin IgA antibody group had less activity and depression,and the body weight was lower than that of the mice in negative anti-gliadin IgA antibody group（P〈0.05）.The results of Balance and coordination test,Open-field test and Forced swimming test showed that the mental status was affected more seriously in positive anti-gliadin IgA antibody group than that in negative group（P〈0.05）.The ratio of the cerebellum to whole brain in positive anti-gliadin IgA antibody group was significantly decreased as compared with negative group（P〈0.05）.Conclusion The immunological reaction induced by gliadin maybe influence the development and function of central nervous system in mice.

关 键 词：谷胶蛋白 免疫反应 中枢神经系统 

分 类 号：R338.2[医药卫生—人体生理学]