复方参草颗粒对大鼠酒精性脂肪肝的拮抗作用  

Antagonistic effect of Compound Shencao Granules on alcoholic fatty liver in rats

作　　者：李艳杰[1] 刘智[2] 于艳华[3] 韩杨[2] 许大艳[1] 

机构地区：[1]长春中医药大学药学院药物化学教研室,吉林长春130117 [2]长春中医药大学基础医学院药理学教研室,吉林长春130117 [3]白城医学高等专科学校药学系药理学教研室,吉林白城137000

出　　处：《吉林大学学报（医学版）》2010年第4期717-720,I0002,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省中医药管理局青年基金资助课题(08sys-019)

摘　　要：目的:探讨复方参草颗粒(CSCG)对酒精性脂肪肝大鼠血清生物化学指标及肝组织形态学变化的影响。方法:60只大鼠随机分为6组,每组10只,即正常对照组、模型组、阳性药脂可清胶囊组及CSCG高、中、低剂量组。正常对照组大鼠喂食普通饲料;其余各组大鼠喂食380mL.L-1酒精及高脂饲料,连续15d,制备高脂模型。第16天起,灌胃给予各实验组大鼠相应药物,连续40d。末次给药12h后腹主动脉取血,检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、谷丙转氨酶(ALT)及谷草转氨酶(AST)含量,计算TC/HDL-C,LDL-C/HDL-C比值及动脉硬化指数(AI);断头处死大鼠,剖取肝脏,行常规HE染色,光学显微镜下观察。结果:与模型组比较,CSCG高剂量组大鼠血清TC,TG,LDL-C、TC/HDL-C、LDL-C/HDL-C及AI均有不同程度的降低(P<0.05或P<0.01),血清HDL-C含量升高(P<0.01),AI下调(P<0.01);光学显微镜下CSCG高、中剂量组大鼠肝组织内脂滴成分明显减少,肝细胞结构及排列明显改善。结论:CSCG对酒精性脂肪肝具有一定的拮抗作用。Objective To investigate the effect of Compound Shencao Granules（CSCG）on biochemistry indicators in serum and the pathologic changes of liver tissue in rats suffered from alcoholic fatty liver.Methods Sixty rats were divided into six groups（n=10）：control group,model group,Zhikeqing Capsule（ZKQC）group,CSCG（high,middle and low doses）groups.The rats in control group were fed with full forage,the rats in the other groups were fed with high fat forage plus 380 mL·L-1 of alcohol for 15 d.After successing for the model preparation,the rats in each group were fed on corresponding drugs for 40 d.12 h after the last administration,the blood samples were collected from abdomen-artery and the contents of total cholesterol（TC）,triglyeride（TG）,low density lipoprotein cholesterol（LDL-C）,high density lipoprotein cholesterol（HDL-C）,alanine aminotransferase（ALT）and aspartate aminotransferase（AST）were detected,TC/HDL-C,LDL-C/HDL-C and atherogenie index（AI）were calculated.The rats were put to death by cutting through the head,and their anatomized livers were dyed with HE and examined by light microscope.Results Compared with model group,the levels of TC,TG,LDL-C,TC/HDL-C,LDL-C/HDL-C and AI in high dose CSCG group were significantly decreased（P〈0.05 or P〈0.01）,HDL-C was increased（P〈0.01）and AI was decreased（P〈0.01）;under hight microscope,the ingredients of lipid droplet in CSCG groups（high and middle doses）were significantly reduced and the hepatic cells were relieved.Conclusion CSCG has antagonistic effect on alcoholic fatty liver in rats.

关 键 词：复方参草颗粒 脂肪肝 酒精性 胆固醇 天冬氨酸氨基转移酶类 丙氨酸转氨酶 大鼠 Wistar 

分 类 号：R285.3[医药卫生—中药学]