HLA-DRB1等位基因与吉林地区汉族人群慢性乙型病毒性肝炎的关联性分析  

Relevance analysis on HLA-DR1 alleles and chronic hepatitis B of Han population in Jilin province

作　　者：韩瑜[1] 马宁[2] 焦立新[1] 林乾飞 姜振宇[2] 

机构地区：[1]吉林省血液中心输血研究所,吉林长春130033 [2]吉林大学第一医院风湿病科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2010年第4期741-744,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(20090454)

摘　　要：目的:探讨HLA-DRB1基因多态性与吉林地区汉族人群慢性乙型肝炎病毒(HBV)感染的相关关系,为慢性乙型病毒性肝炎的治疗和预防提供新思路。方法:采用聚合酶链反应-序列特异性寡核苷酸探针技术(PCR-SSOP)对398例汉族慢性乙型病毒性肝炎患者及838名汉族健康献血者(对照组)进行HLA-DRB1等位基因分型比较。结果:慢性乙型肝炎组HLA-DRB1*03、09等位基因表达频率分别为6.28%和19.60%,明显高于健康对照组(3.58%和12.17%;P=0.031,OR=1.805;P=0.001,OR=1.759);慢性乙型肝炎组HLA-DRB1*04、10和13等位基因表达频率分别为6.28%、0.50%和3.27%,明显低于健康对照组(11.34%、2.39%和5.97%;P=0.005,OR=0.524;P=0.019,OR=0.207;P=0.044,OR=0.523);而所检测HLA-DRB1座位的其他等位基因表达频率与健康对照组比较差异无显著性(P>0.05)。结论:HLA-DRB1*03、09可能是吉林地区汉族慢性乙型病毒性肝炎患者易感基因,HLA-DRB1*04、10、13可能是保护基因。Objective To explore the correlation between the HLA-DRB1 alleles and the chronic hepatitis B of the Han population in Jilin province,and provide a new way for treatment and prevention of chronic hepatitis B.Methods HLA-DRB1 alleles in 398 patients with chronic hepatitis B and 838 healthy controls were analyzed by using the polymerase chain reaction-sequence specific oligonucleotide probe（PCR-SSOP）technique.Results The frequencies of HLA-DRB103,09 alleles in chronic hepatitis B group were higher than those in healthy control group（6.28% vs 3.58%,P=0.031,OR=1.805;19.60% vs 12.17%,P=0.001,OR=1.759）.The frequencies of HLA-DRB104,10 and 13 alleles in chronic hepatitis B group were lower than those in healthy control group（6.28% vs 11.34%,P=0.005,OR=0.524;0.50% vs 2.39%,P=0.019,OR=0.207;3.27% vs 5.97%,P=0.044,OR=0.523）.There were no significant differences in the frequencies of the other HLA-DRB1 alleles between chronic hepatitis B group and healthy control group（P〉0.05）.Conclusion The HLA-DRB103,09 alleles may be susceptible genes for chronic hepatitis B,and HLA-DRB104,10,13 alleles may be protecting gene for chronic hepatitis B of Han population in Jilin province.

关 键 词：慢性乙型病毒性肝炎 人类白细胞抗原 PCR-SSOP 汉族 

分 类 号：R512.6[医药卫生—内科学]