自拟川黄三七汤防治自体移植静脉再狭窄的实验研究  

Study on the Prevention of Autologous Vein Graft Restenosis by Chuanhuang-sanqi Medicinal Broth

作　　者：郭明兴[1] 

机构地区：[1]山东省卫生教育培训中心,250014

出　　处：《中国医药指南》2010年第23期13-15,共3页Guide of China Medicine

摘　　要：目的研究自拟川黄三七汤对兔颈外静脉移植至股动脉后再狭窄的防治作用,并探讨可能的机制。方法 50只新西兰大白兔,随机分为实验组(川黄三七组)、对照组(生理盐水组)。实验组术前3d开始采用灌胃法给予川黄三七汤,对照组给与等量生理盐水,1次/d,直至术后取材。所有动物于术日建立自体静脉旁路移植模型:利用显微外科技术将颈外静脉端侧吻合于股动脉,于术后4周取血管桥标本,同时取0.5cm移植静脉作为正常静脉参照。光镜下观察、测定移植静脉内膜中膜厚度变化。扫描电镜观察近吻合口处和中央部静脉桥的管腔狭窄程度,管腔增生面积与管腔面积比(S1/S2),统计学分析各组间差异。结果移植4周后,各组静脉均有内膜及中膜较正常颈静脉明显增厚表现,实验组内膜及中膜增厚较对照组轻。两组静脉桥的管腔狭窄程度进行比较存在明显差异。结论川黄三七汤能抑制动脉化静脉内膜及中膜增厚,有减轻桥静脉狭窄的作用。Objective To study the protective effects of Chuanhuang-sanqi medicinal broth on the external jugular vein after being transplanted into femoral artery and explore its possible mechanism. Methods Autogenous vein grafts model was established in 50 rabbits. Interposition jugular vein were transplaced into carotid artery of the rabbits by microsurgical technique. Animals received Chuanhuang-sanqi medicinal broth. The control group given normal saline by oral gavage 72 hours before and then daily after surgery. Interposition jugular vein were transplanted into femoral arteries of the rabbits by microsurgical technique. grafting veins weresampled after 4 weeks,At the same time take normal vein did not refer to the opposite side of vein graft. The veins were cut into 2 segments. One was for endothelial integrity grading by SEM examination. The other was interposed between contralateral common carotid arteries. The vein grafts were harvested at 2 weeks after grafting. Neintimal square-to-luminal square ratio（S1/S2）was measured with a computerized image analysis system. Result The stenosis of grafted veins： 4 weeks later, the jugular veins′ intima/media membrane of all the operative groups were signifi cantly thicker than The normal control group. There were signifi cant differences between every two group（P〈0.05）. Conclusion Chuanhuang-sanqi medicinal broth can lessen the thickness of arterialized vein′s intima/media membrane and lighten the stenosis of the bridging vein.

关 键 词：内膜增生 细胞增殖 静脉移植 川黄三七汤 

分 类 号：R543.6[医药卫生—心血管疾病]