靶向乙型肝炎病毒X区的siRNAs对HepG2.2.15细胞HBV复制的抑制作用  

Inhibition of hepatitis B viral replication in HepG2215 cells by siRNAs targeting hepatitis B virus X gene

作　　者：纵书芳[1] 陈勇[2] 王莉娟[2] 刘兴祥[2] 徐云芳[2] 

机构地区：[1]徐州市徐州医学院 [2]淮安市第四人民医院肝病研究所

出　　处：《实用肝脏病杂志》2010年第4期253-255,共3页Journal of Practical Hepatology

基　　金：淮安市科技发展项目(编号:HAG0801)

摘　　要：目的观察siRNA对HBV基因表达和复制的抑制情况。方法针对HBVX区设计并化学合成4条siRNAs,观察在不同浓度下对HepG2.2.15细胞HBV复制的抑制作用。结果 siRNA在30nmoL/L浓度时,4种siRNA对HepG2.2.15细胞HbsAg和HBeAg无明显的抑制作用(P>0.05);在60nmoL/L和90nmoL/L浓度下,siR-NA-1和siRNA-4有明显的抑制作用(P<0.05),他们对HBsAg和HBeAg的抑制率分别为41%和43%;siRNA能降低HepG2.2.15细胞HBVDNA的拷贝数。结论化学合成的siRNAs可以有效地抑制HBV基因的表达和复制。Objective To observe the inhibition of hepatitis B viral replication in HepG2215 cells by siRNAs targeting hepatitis B virus X gene. Methods Four siRNAs against the X region of hepatitis B virus were chemically synthesized,and then were subjected to HepG2.2.15 cells by liposome-mediated transfection. The HBsAg and HbeAg in the supernatant were assayed by ELISA and HBV DNA by RT-PCR. Results At 30nmoL/L, the four siRNA had no obvious suppression in HBeAg and HbsAg expression （P〉0.05）,while at the concentration of 60nmoL/L and 90nmoL/L,the inhibition of siRNA-1 and siRNA-4 were statistically significant with the inhibition rates of 41% and 43%;the HBV DNA replication was obviously reduced. Conclusion The chemically synthesized siRNAs can effectively inhibit hepatitis B viral replication in vitro.

关 键 词：HEPG2.2.15细胞 乙型肝炎病毒 RNA干扰 

分 类 号：R512.62[医药卫生—内科学]