水稻抗白叶枯病基因Xa23分子标记的优化和验证  

Optimization and Verification of Molecular Markers for Rice Bacteial Blight Resistance Gene Xa23

作　　者：高利军[1,2] 高汉亮[1] 李容柏[1,2] 李道远[1] 周萌[1] 颜群[1] 周维永[1] 张晋[1] 邓国富[1,2] 

机构地区：[1]广西农业科学院,南宁530007 [2]广西作物遗传改良生物技术重点开放实验室,南宁530007

出　　处：《分子植物育种》2010年第4期660-664,共5页Molecular Plant Breeding

基　　金：国家863计划子课题(2006AA100101-2);广西科技创新能力与条件建设项目(桂科能0815011-1-1-9);广西科技基础条件平台建设项目(09-007-08)共同资助

摘　　要：本研究对抗稻白叶枯病基因Xa23的标记03STS1进行改良和优化,设计分子标记命名为M-Xa23。以72个水稻亲本以及含抗性基因Xa23的CBB23与感病恢复系金刚30杂交的F2代分离群体为材料,并用白叶枯病Ⅶ型菌接种,结合抗病表型和标记结果,分析了M-Xa23的特异性和标记选择的准确性。实验结果表明:M-Xa23在抗病品种CBB23中扩增出200bp的特性条带,在其它71个水稻亲本中扩增出346bp的条带。144株F2分离群体中,109株表现抗病,35株表现感病,与标记M-Xa23检测的F2群体的基因型完全吻合,结果证明标记M-Xa23特异性强,能准确用于抗病基因Xa23的辅助选择。In the present study,the molecular marker of bacterial blight（BLB） resistance gene Xa23,03STS1,was improved and optimized.The new marker obtained was designated as M-Xa23.Inoculating Ⅶ-type of BLB pathogen to 72 parents and a F2 segregating population from a cross between CBB23 containing gene Xa23 and susceptible variety Jingang 30,the specificity and veracity of the marker M-Xa23 were analyzed.Experimental results showed that M-Xa23 amplified a specific 200 bp band in resistant variety CBB23 and a 346 bp band in the remaining 71 parents（susceptible varieties）.Among the 144 F2 plants of F2 segregating population,109 plants performed disease resistance and 35 plants showed susceptibility,which was completely in accordance with the genotype results detected by marker M-Xa23.Marker M-Xa23,thereby,demonstrated strong specificity and veracity in marker-assisted selection for BLB resistance gene Xa23.

关 键 词：水稻 白叶枯病 分子标记 优化和验证 

分 类 号：S511[农业科学—作物学]