强优势杂交棉产量性状的QTL定位  

QTL Mapping of Yield Traits in High Advantage Combination of Cotton

作　　者：李昆[1] 杨代刚[1] 马雄风[1] 周晓箭[1] 王海凤[1] 孟清芹[1] 裴小雨[1] 

机构地区：[1]中国农业科学院棉花研究所,农业部棉花遗传改良重点实验室,安阳455000

出　　处：《分子植物育种》2010年第4期673-679,共7页Molecular Plant Breeding

基　　金：杂交棉育种技术研究与新品种选育项目(2006BAD01A05-02)资助

摘　　要：本研究以中9018自交系为母本以及中棉所45自交系为父本进行杂交,构建了219个F2:3家系,将其分成两份后分别种植于河南省安阳和安徽省望江,每个点分别设置了三个重复。用6753对引物筛选亲本多态性,获得了193个多态位点并进行标记间连锁分析,其中的127个位点构建了一张包括33个连锁群,全长为1396.2cM的陆地棉品种间分子标记遗传图谱。另外利用基于混合线性模型的QTLNet-work2.0软件对产量相关性状进行QTL分析,共检测到5个加性QTL和10对上位性QTL。这些结果为进一步的QTL精细定位以及棉花杂种优势利用奠定了基础,并可以此为参考选择部分材料供后续研究使用。We used 9018 inbred lines as maternal parent and CRI45 as paternal parent,respectively,to construct the F2：3 mapping population.The F2：3 mapping population includes 219 lines which was divided into two parts：one was planted in Anyang,Henan,and the other was planted in Wangjiang,Anhui,3 blocks in every environment.In this research we identified 193 polymorphic loci with 6 753 pairs of SSR primers and than the linkage test indicated that 127 loci could be mapped to 33 linkage groups which had a whole genetic distance of 1 396.2 cM of upland cotton.The QTL analyses for yield-related traits in F2：3 populations showed that five additive QTLs and ten pairs of epistatic QTLs were detected by employing the software QTLNet-work version 2.0 based on the mixed linear model.The results may lay a base for subtle QTL mapping,utilizaion of cotton heterosis,and materials choosing for subsequent research.

关 键 词：强优势杂交棉 产量性状 QTL定位 

分 类 号：S562[农业科学—作物学]