银杏内酯B的提取、分离与纯化研究  

Extraction,isolation and purification for Ginkgolide B

作　　者：章晨峰[1,2] 李明慧[2] 唐云[2] 张艳海[2] 施敏[2] 盛龙生[1] 

机构地区：[1]中国药科大学,江苏南京210009 [2]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《中国中药杂志》2010年第15期1961-1964,共4页China Journal of Chinese Materia Medica

基　　金：国家863计划课题(2002AA2Z3215)

摘　　要：目的:建立1种较简单的从银杏叶中提取、分离和纯化银杏内酯B的方法。方法:以银杏内酯B得率为指标,对银杏内酯B的提取、分离和纯化工艺进行优选。结果:银杏叶用70%乙醇提取3次,浓缩至无醇味,加水稀释至每1mL含生药材0.1g,HPD-450大孔吸附树脂吸附,20%乙醇除杂,80%乙醇洗脱、浓缩、结晶,异丙醇重结晶,得到纯度为95%的银杏内酯B结晶。结论:该工艺稳定、简便易行,适合工业化大生产。Objective：To establish a simple extraction,isolation and purification method for Gingkolide B from Ginkgo leaf.Method：The optimum conditions of extraction,isolation and purification were studied by taking the transfer rate of Ginkgolide B as index.Result：Ginkgo leaf was extracted with 70% ethanol for three times,the extracts were concentrated to remove ethanol and diluted by water till the crude drug density reached 0.1 g·mL-1.The dilution was adsorbed with HPD-450 macroporous resin.The impurities were eluted with 20% ethanol and Ginkgolide B was eluted with 80% ethanol.Then the 80% ethanol eluant was concentrated and crystallized.Finally the crude crystals were recrystallized with isopropanol.The purity of the Ginkgolide B recrystallization was 95%.Conclusion：The process was stable and easy to operate,which was suited to industrialized production.

关 键 词：银杏内酯B 醇提水沉 树脂吸附 重结晶 

分 类 号：R284.2[医药卫生—中药学]