桐叶千金藤中莲花烷类生物碱的研究  

Hasubanan type alkaloids in Stephania hernandifolia

作　　者：唐丽佳[1,2] 张援虎[1] 郝小燕[2] 何丽[1] 宋少辉[1] 郝小江[1] 杨小生 

机构地区：[1]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002 [2]贵阳医学院药学院,贵州贵阳550004

出　　处：《中国中药杂志》2010年第15期1973-1977,共5页China Journal of Chinese Materia Medica

基　　金："贵州省民族药活性筛选中心"建设(黔科合农字[2006]5041号);贵州省民族药公共技术服务平台(黔发改投资[2007]1712号)

摘　　要：目的：研究桐叶千金藤Stephania hernandifolia中的莲花烷类生物碱。方法：桐叶千金藤全草用95%乙醇溶液加热回流提取,减压回收乙醇,浓缩液加水稀释后经酸碱处理,石油醚、氯仿萃取,得生物总碱。生物总碱利用正相硅胶、反相Rp-18、微孔吸附树脂CHP20P、Sephadex LH-20等柱色谱方法分离和纯化,通过理化性质和光谱数据进行结构鉴定。结果：从桐叶千金藤中分离得到9个莲花烷类生物碱,分别为aknadinine（1）,longanone（2）,stephasunoline（3）,N-methylstephuline（4）,epistephamiersine（5）,prostephabyssine（6）,aknadilactam（7）,dihydroepistephamiersine（8）,hasubanonine（9）。结论：化合物2~8为首次从该植物中分离得到。Objective：To study the hasubanan type alkaloids in Stephania hernandifolia.Method：The dried herbs of S.hernandifolia.were extracted with 95% ethanol.After removal of the solvent,the residue was first partitioned between acid water and petroleum ether,then the aqueous layer was basified and extracted with chloroform to obtain crude alkaloids.Column chromatograghic methods with on silica gel,Rp-18,MCI CHP 20P,Sephadex LH-20 were applied for the isolation and purification of the crude alkaloid fraction.The structures were elucidated by their physicochemical properties and spectral data.Result：Nine hasubanan type alkaloids were obtained and identified as aknadinine（1）,longanone（2）,stephasunoline（3）,N-methylstephuline（4）,epistephamiersine（5）,prostephabyssine（6）,aknadilactam（7）,dihydroepistephamiersine（8）,hasubanonine（9）.Conclusion：Compounds 2-8 were isolated from this plant for the first time.

关 键 词：桐叶千金藤 莲花烷类生物碱 

分 类 号：R284.1[医药卫生—中药学]