N-苯甲酰-L-精氨酸乙酯测定水蛭提取物生物活性  

Determination of biological activity of extract from hirudo by N-benzoyl-L-arginine ethyl ester

作　　者：关世侠[1] 彭灿[1] 李海刚[1] 许淑芹[1] 袁中文[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《中国中药杂志》2010年第15期1990-1992,共3页China Journal of Chinese Materia Medica

基　　金：广东省自然科学基金项目(8151040701000058)

摘　　要：目的:建立水蛭提取液生物活性测定方法。方法:用N-苯甲酰-L-精氨酸乙酯(BAEE)为底物,通过测定反应体系中未被水蛭提取物抑制的凝血酶的量,间接测定水蛭提取物抗凝血酶的生物活性。结果:水蛭提取物的生物活性测定曲线线性关系良好,回归方程为Y=0.0014X+0.0196,r=0.9994。结论:本方法于30℃条件下反应25min进行测定,日间、日内精密度分别为2.6%和1.9%,适宜作为提取物生物活性测定方法。As a potent anticoagulant,leech a traditional Chinese medicine,has become increasing topics.Hirudin,which is the primary effective component in leech,is a specific and efficient inhibitor of thrombin,mainly used in prevention and treatment of thrombus on the clinic practice.However,there is still no accurate and convenient method reported about the determination of it＇s biological activity.This paper reported a method for the determination of the biological activity the of extract from hirudo.The extra thrombin,which was not inhibited by hirudin in the extract from hirudo,reacted with N-benzoyl-L-arginine ethyl ester and was determined.The biological activity of the hirudo extract was determined,indirectly.The linear of calibration curve and accuracy were both perfect,the method was accurate and reliable.

关 键 词：水蛭提取物 生物活性 N-苯甲酰-L-精氨酸乙酯 

分 类 号：R284.1[医药卫生—中药学]