痛泻要方对内脏高敏感大鼠脑、脊髓CRF表达的影响  

Influence of Tongxie prescription on CRF expression in spinal cord and brain of hypersensitive viscera rats

作　　者：晁冠群[1] 吕宾[2] 孟立娜[2] 张烁[2] 张璐[2] 郭赟[3] 

机构地区：[1]浙江大学医学院附属邵逸夫医院,浙江杭州310006 [2]浙江中医药大学附属第一医院,浙江杭州310006 [3]杭州市第二医院,浙江杭州310006

出　　处：《中国中药杂志》2010年第15期2012-2016,共5页China Journal of Chinese Materia Medica

摘　　要：目的:研究内脏高敏感大鼠的脊髓、下丘脑、第三脑室下侧促肾上腺皮质激素释放因子(corticotropin releasingfactor,CRF)的分布和表达,探讨CRF在肠易激综合征(irritable bowel syndrome,IBS)内脏高敏感信号传导通路中的机制以及痛泻要方对IBS起效的可能作用机制。方法:清洁级成年雌性SD大鼠40只,随机分3组,模型一组腹腔注射鸡卵清蛋白致敏,模型二组条件刺激和非条件刺激轮替致敏,评估肠道敏感性后均随机分成2组,5组分别予生理盐水或药物,灌胃4周,取脑、脊髓进行免疫组化,观察CRF的分布和表达情况。结果:痛泻要方组内脏敏感性较模型组明显降低(P<0.01)。免疫组化显示大鼠下丘脑、第三脑室下侧、脊髓腰膨大部可见CRF的明显表达,CRF阳性指数模型组较空白组明显增高(P<0.01),治疗组较模型对照组明显降低(P<0.01),治疗组较空白组明显增高(P<0.01)。结论:2种不同刺激诱发的大鼠模型均出现内脏高敏感状态,与应激相关的CRF在下丘脑、第三脑室下侧以及脊髓的表达上调,提示CRF在内脏刺激信号的传入过程中起重要作用。应用痛泻要方可以降低其表达程度,这可能是降低大鼠内脏高敏感的机制之一。Objective：To investigate the distribution and expression of corticotropin releasing factor（CRF）in spinal cord,hypothalamus and third ventricle of cerebrum,of the hypersensitive viscera rats,and to research on the mechanism of CRF in the hypersensitive vicera signal conduction pathway in IBS（irritable bowel syndrome）and to investigate possible active mechanisms of Tongxie prescription on IBS.Method：Forty SD rats were divided randomly into three groups.The rats of model No.1 were sensitized by injecting egg albumin into abdominal cavity.The rats of model No.2 were sensitised by conditional stimulus and unconditional stimulus.The two model groups were both divided randomly into two groups.The five groups were given intragastric administration with Tongxie prescription or normal saline for four weeks.Then quantitative analysis of CRF in the lumbosacral spinal cord and brain of rats were achieved by immunohistochemical method and computerized image system.Result：The sensitivity of the groups being treated with Tongxie prescription were much lower than the model groups（P〈0.01）.The immunohistochemical method showed that CRF was expressed in the lumbar intumescentia of spinal cord,hypothalamus and the diaphragmatic surface of third ventricle of cerebrum.The CRF positive index of the model groups was higher than that of the blank group（P〈0.01）.The CRF positive index of the healing groups was lower than that of the model groups（P〈0.01）.The CRF positive index of the healing groups was higher than that of the blank group（P〈0.01）.Conclusion：The two model rats evoked by two different stimulation both appeared visceral hypersensitivity state.CRF is related to stress.The increase of CRF expression in the lumbar intumescentia of spinal cord,hypothalamus and the diaphragmatic surface of third ventricle of cerebrum showed that CRF is critical to the introduce of stimulus signal of vicera.Tongxie prescription can significantly decrease CRF expression.This is one mechanism to decrease sensitivi

关 键 词：痛泻要方 CRF 内脏高敏感 下丘脑 第三脑室 脊髓 

分 类 号：R285.5[医药卫生—中药学]