持续性术后痛大鼠脊髓星形胶质细胞的活化  

Glial activation in spinal cord in a rat model of persistent postoperative pain

作　　者：范素贞[1] 胡兴国[2] 曾因明[1] 

机构地区：[1]徐州医学院江苏省麻醉与镇痛应用技术重点实验室,221002 [2]湖南省桃源县人民医院麻醉科

出　　处：《中华麻醉学杂志》2010年第5期562-564,共3页Chinese Journal of Anesthesiology

基　　金：湖南省科技计划一般项目资助课题（2009SK3018）

摘　　要：目的 探讨持续性术后痛大鼠脊髓背角星形胶质细胞的活化.方法 成年雄性SD大鼠48只,体重200～250 g,随机分为2组（n=24）：假手术组和模型组.模型组采用皮肤肌肉切口牵拉术制备持续性术后痛模型,假手术组仅暴露内收肌,不牵拉皮肤肌肉组织.分别于模型制备前（基础状态）、制备后1、3、12、22和32 d时测定大鼠机械缩足阈值（MWT）.各时点MWT测定结束后随机取4只大鼠,处死后采用免疫组织荧光法测定脊髓胶质纤维酸性蛋白（GFAP）的表达水平.结果 与基础值比较,假手术组各时点MWT和脊髓GFAP表达水平差异无统计学意义（P＞0.05）,模型组模型制备后1～22 d MWT降低,12 d时最低,模型制备后3～22 d脊髓GFAP表达上调,12 d时达峰值（P＜0.05或0.01）;与假手术组比较,模型组MWT降低,脊髓GFAP表达上调（P＜0.05）.结论 大鼠持续性术后痛与脊髓星形胶质细胞的活化有关.Objective To investigate the glial activation in the spinal cord in a rat model of persistent postoperative pain. Methods Forty-eight adult male SD rats weighing 200-250 g were randomly divided into 2 groups （ n = 24 each）： group Ⅰ sham operation （group S） and group Ⅱ persistent postoperative pain. Persistent postoperative pain was evoked by skin/muscle incision and retraction （SMIR） as described by Flatters. Pawwithdrawal threshold to yon Frey hair stimulation was measured before operation （baseline） and at 1, 3, 12, 22and 32 d after establishment of the model. Four animals were sacrificed at each time point and lumbar segment of the spinal cord was removed for determination of expression of glial fibrillary acidic protein （GFAP） in the astrocytes by immunofluorescence histo-chemistry assay. Results The mechanical threshold started to decrease at 1 d after establishment of the model, and peaked at 12 d after establishment of the mode. Immunofluorescence histochemistry assay demonstrated that GFAP expression in the dorsal horn was significantly increased at 3 d after estabhshment of the model and reached the peak at 12 d and was maintained at the high level until 22 d after establishment of the model. Conclusion Glial activation is involved in the mechanism of persistent postoperative pain evoked by SMIR.

关 键 词：疼痛 手术后 星形细胞 脊髓 

分 类 号：R614[医药卫生—麻醉学]