依达拉奉对大鼠心肌缺血再灌注诱发肺损伤的影响  

Effects of edaravone on lung injury induced by myocardial ischemia-reperfusion in rats

作　　者：张玮玮[1] 郭永清[1] 余淑珍[1] 荆建敏[1] 卫建峰[1] 

机构地区：[1]山西省人民医院麻醉科,太原市030012

出　　处：《中华麻醉学杂志》2010年第5期608-611,共4页Chinese Journal of Anesthesiology

基　　金：山西省卫生厅科技攻关计划项目（200948）

摘　　要：目的 探讨依达拉奉对大鼠心肌缺血再灌注诱发肺损伤的影响.方法 健康清洁级雄性Wistar大鼠24只,体重250～300 g,随机分为4组（n=6）：假手术组（S组）、心肌缺血再灌注组（IR组）和不同剂量依达拉奉组（E1组和E2组）.IR组、E1组和E2组采用结扎冠状动脉左前降支（LAD）45 min,再灌注3 h的方法制备心肌缺血再灌注模型.S组仅LAD下穿线不结扎;IR组阻断LAD45 min后再灌注3 h;E1组和E2组分别于再灌注前1 min经右股静脉注射依达拉奉3或10 mg/kg.于再灌注3 h时放血处死大鼠,取肺组织、支气管肺泡灌洗液和动脉血样,测定血清肌酸激酶同工酶（CK-MB）活性,计算肺通透性指数（PPI）,采用Western blot法检测肺组织β-防御素-2（BD-2）和TNF-α蛋白的表达,PCR法测定肺组织BD-2 mRNA表达.结果 与S组比较,IR组、E1组和E2组血清CK-MB活性和PPI升高,肺组织BD-2 mRNA、BD-2和TNF-α蛋白表达上调（P＜0.01）.与IR组比较,E1组和E2组血清CK-MB活性和PPI降低,肺组织BD-2 mRNA、BD-2和TNF-α蛋白表达下调（P＜0.01）.与E1组比较,E2组血清CK-MB活性和PPI降低,肺组织BD-2 mRNA、BD-2和TNF-α蛋白表达下调（P＜0.01）.结论 依达拉奉可减轻大鼠心肌缺血再灌注诱发的肺损伤,其机制不仅与清除氧自由基有关,还与抑制肺组织炎性反应有关.Objective To investigate the effects of edaranvone on lung injury induced by myocardial ischemia-reperfusion （I/R） in rats. Methods Twenty-four male Wistar rats weighing 250-300 g were randomly assigned to one of 4 groups （ n = 6 each）： group Ⅰ sham operation （group S）; group Ⅱ myocardial I/R and group Ⅲ and Ⅳ different doses of edaravone （ group E1, E2 ）. The animals were anesthetized, intubated and mechanically ventilated. In group Ⅱ-Ⅳ myocardial I/R was induced by occlusion of left anterior descending coronary artery for 45 min followed by 3 h reperfusion. In group Ⅲ and Ⅳ edavarone 3 and 10 mg/kg was administered via right femoral vein at 1 min before reperfusion respectively. The animals were sacrificed by exsanguination at the end of 3 h reperfusion. Blood was collected for determination of serum CK-MB activity and total protein content. The left lung was lavaged and the broncho-alveolar lavage fluid （BALF） was colleted for determination of protein content. Pulmonary permeability index （PPI） was calculated. The lung tissue was obtained for determination of BD-2 mRNA and protein and TNF-α expression. Results The serum CK-MB activity, PPI,BD-2 mRNA and protein and TNF-α expression were significantly higher in group I/R, E1 and E2 than in group S,but significantly lower in group E1 and E2 than in group I/R and in group E2 than in group E1. Conclusion Edaravone can reduce myocardial I/R-induced lung injury by scavenging oxygen free radicals and inhibiting inflammatory response of lung tissues in rats.

关 键 词：自由基清除剂 心肌再灌注损伤 呼吸窘迫综合征 成人 

分 类 号：R285.5[医药卫生—中药学]