编码harpin_(Xoo)蛋白的基因hpa1_(Xoo)转化菊花增强对蚜虫的抗性  

Chrysanthemum Transformed with hpa1_(Xoo) Enhanced Resistance to Aphid

作　　者：柴一秋[1,2] 陈官菊[1,2] 李明[1] 潘小玫[1] 邵敏[1] 王金生[1] 

机构地区：[1]南京农业大学植物保护学院/农业部病虫监测与治理重点开放实验室,南京210095 [2]浙江省亚热带作物研究所,温州325005

出　　处：《中国生物防治》2010年第3期280-286,共7页Chinese Journal of Biological Control

基　　金：国家自然科学基金(30230240);国家重点基础研究发展规划资助项目(G2006CB101902)

摘　　要：将水稻白叶枯病原菌Xanthomonas oryzae pv.oryzae编码harpinXoo蛋白的hpa1Xoo基因构建植物重组表达质粒pCAMBIA3300-hpa1Xoo-bar,由根癌农杆菌Agrobacterium tumefa-ciensLBA4404介导,叶盘法转化寒菊QX-077,获得了草丁膦转基因寒菊株系。经PCR、Southernblot和RT-PCR检测证明hpa1Xoo已整合到寒菊基因组中。在转基因株系的叶片和植株上抗虫性鉴定表明,转基因寒菊显著抑制桃蚜Myzus persicae的繁殖(t<0.01)。RT-PCR表明转基因植株中hpa1Xoo能够在转录水平正常表达。hpa1Xoo转化寒菊可以正常表达并提高寒菊对蚜虫的抗性。The gene hpa1Xoo encoding harpinXoo protein derived from Xathomonas oryzae pv.oryzae was inserted into transgenic vector pCAMBIA3300-bar.The leaf discs from chrysanthemum variety ＇Han jv＇ QX-077 were infected by Agrobacterium tumefaciens strain LBA4404/pCAMBIA3300-hpa1Xoobar.Basta-resistant green shoots were obtained.PCR,Southern blot and RT-PCR analyses confirmed successful integration of the foreign gene into the genome of the ＇Han jv＇ QX-077.Resistance bioassays of the leaves and plants of transgenic lines revealed that the transgenic plants could significantly inhibit the proliferation of Myzus persicae（t0.01）.RT-PCR analysis confirmed that hpa1Xoo had expressed in transgenic ＇han jv＇ plants at transcription level.Transformed chrysanthemum with hpa1Xoo gene could enhance the capability of aphid resistance.

关 键 词：hpa1Xoo 转基因菊花 蚜虫抗性 水稻黄单胞菌 

分 类 号：Q786[生物学—分子生物学] S436.8[农业科学—农业昆虫与害虫防治]