分子标记辅助选择聚合水稻暗胚乳突变基因Wx-mq和抗条纹叶枯病基因Stv-b^i  

Pyramiding of Translucent Endosperm Mutant Gene Wx-mq and Rice Stripe Disease Resistance Gene Stv-b^i by Marker-Assisted Selection in Rice (Oryza sativa)

作　　者：姚姝[1,2] 陈涛[1] 张亚东[1] 朱镇[1] 赵凌[1] 赵庆勇[1] 周丽慧[1] 王才林[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心,江苏南京210014 [2]云南农业大学农学与生物技术学院,云南昆明650201

出　　处：《中国水稻科学》2010年第4期341-347,共7页Chinese Journal of Rice Science

基　　金：国家转基因生物新品种培育科技重大专项资助项目(2008ZX08001-006;2009ZX08001-019B);农业部行业科研专项资助项目(nyhyzx07-001-006);江苏省重大科技支撑计划资助项目(BE2008354);江苏省农业科技自主创新基金资助项目(CX[09]634)

摘　　要：以江苏高产粳稻品种武运粳7号为母本,具有条纹叶枯病抗性基因Stv-bi和暗胚乳突变基因Wx-mq的日本粳稻品种关东194作父本配制杂交组合进行聚合育种。利用与Stv-bi基因共分离的SCAR标记及与暗胚乳突变基因Wx-mq紧密连锁的CAPS功能标记对其分离世代进行标记位点的检测,结合田间多代选育、抗性鉴定和籽粒胚乳外观鉴定,将条纹叶枯病抗性基因Stv-bi和暗胚乳突变基因Wx-mq同时转育到高产品种中,筛选、培育出优质、抗病、高产且农艺性状优良的水稻新品系宁9108。利用与水稻优质及抗病基因紧密连锁的分子标记,不仅初步建立了优质、抗病育种的分子标记辅助选择体系,也为水稻优质、抗病、高产育种提供了一种简单、快捷的选择方法和重要的中间材料。A high yielding japonica rice variety,Wuyunjing 7,bred in Jiangsu Province,China as female parent was crossed with the Japanese rice variety Kanto 194,which carries the rice stripe disease resistance gene Stv-bi and translucent endosperm mutant gene Wx-mq.From F2 generations,a SCAR （sequence characterized amplified region） marker tightly linked with Stv-bi and a CAPS （cleaved amplified polymorphic sequence） marker for Wx-mq were used for marker-assisted selection.Finally,a new japonica rice line,Ning 9108,with excellent agronomic traits was bred by multi-generational selection on stripe disease resistance and endosperm appearance.The utilization of the markers from genes related to rice quality and disease resistance was helpful not only for establishing a molecular marker-assisted selection system of high-quality and disease resistance in rice,but also for providing important intermediate materials and rapid selection method for good quality,disease resistance and high yield in rice breeding.

关 键 词：水稻 暗胚乳突变基因 抗条纹叶枯病基因 分子标记辅助选择 聚合育种 

分 类 号：S511[农业科学—作物学]