两个水稻抗褐飞虱隐性基因的遗传分析与初步定位  

Genetic Analysis and Preliminary Mapping of Two Recessive Resistance Genes in Rice to Brown Planthopper,Nilaparvata lugens

作　　者：侯丽媛[1,2] 于萍[1] 徐群[1] 袁筱萍[1] 余汉勇[1] 王一平[1] 王彩红[1] 万国[1,2] 彭锁堂[2] 魏兴华[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,浙江杭州310006 [2]山西农业大学农学院,山西太谷030801

出　　处：《中国水稻科学》2010年第4期367-371,共5页Chinese Journal of Rice Science

基　　金：中央级公益性科研院所专项资金资助项目(2009RG001-3);农业部农业野生植物资源保护专项资助项目;浙江省农业重大科技项目(2007C12073)

摘　　要：选用抗褐飞虱的海南普通野生稻渗入系WB01与感虫品种9311杂交,构建F2群体。采用141个具有多态性的SSR标记对303个F2:3株系进行分析,并应用MapMaker/EXP3.0和Windows QTL Cartographer2.0对水稻抗褐飞虱的数量性状基因座进行检测和遗传效应分析。共检测到2个抗性QTL,分别位于第4和第8染色体上,LOD值分别为2.92和3.15,贡献率分别为11.3%和14.9%。An F2 population was derived from the cross of the brown planthopper resistant line WB01,an introgression line of common wild rice （Oryza rufipogon Griff.） from Hainan Province,China and the susceptible indica cultivar 9311.The population with 303 lines was genotyped using 141 simple sequence repeat （SSR） markers and used for gene mapping.Two softwares,MapMaker/Exp 3.0 and Windows QTL Cartographer 2.0 were applied to detect QTLs.Totally,two QTLs on chromosomes 4 and 8 were detected.Each of these QTLs individually explained 11.3% and 14.9% of the phenotypic variations,respectively.

关 键 词：褐飞虱 普通野生稻 抗性基因 数量性状基因座 基因定位 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]