水稻光温敏雄性核不育系广占63S不育基因PTGMS2-1的遗传分析与分子定位  

Genetic Analysis and Molecular Mapping of a Photoperiod-Thermo-Sensitive Genic Male Sterile Gene (PTGMS2-1) in Rice Line Guangzhan 63S

作　　者：王宝和[1] 徐建军[2] 吴银慧[2] 朱金燕[2] 李生强[2] 周勇[2] 程小涛[2] 梁国华[2] 

机构地区：[1]江苏里下河地区农业科学研究所,江苏扬州225007 [2]扬州大学江苏省作物遗传生理国家重点实验室培育点/植物功能基因组学教育部重点实验室,江苏扬州225009

出　　处：《中国水稻科学》2010年第4期429-432,共4页Chinese Journal of Rice Science

基　　金：江苏省六大人才高峰资助项目(06-C-011);国家973计划资助项目(2005CB120807)

摘　　要：水稻光温敏雄性核不育系广占63S的不育基因来源于光敏核不育系农垦58S,其育性转换表现为温敏型,是迄今两系杂交水稻中应用最广泛的籼型光温敏核不育系之一。对广占63S/旱1587组合的F2分离群体进行不育基因的遗传分析,结果表明广占63S的不育性受1对隐性不育基因控制。利用SSR和InDel分子标记技术,结合BSA法,以广占63S与旱1587组合的F2分离群体为材料,将不育基因(暂命名为PTGMS2-1)定位于第2染色体标记S2-4与S2-27之间物理距离390kb的区间内,与两标记间的遗传距离分别为0.5和1.1cM,与标记S2-24共分离。Guangzhan 63S,a photoperiod-thermo-sensitive genic male sterile （PTGMS） line,was one of the most widely used PTGMS lines in two-line indica hybrid rice breeding system in China.Genetic analysis indicated that the sterility of Guangzhan 63S was controlled by a single recessive gene using the F2 population derived from a cross between the Guangzhan 63S and Han 1587.Molecular mapping was performed on the F2 mapping population using SSR and InDel markers and bulked-segregant analysis（BSA）.The PTGMS2-1 was restricted to a 390-kb region on chromosome 2 between the markers S2-4 and S2-27,at genetic distances of 0.5 cM and 1.1 cM,respectively,and co-segregated with S2-24.

关 键 词：光温敏雄性不育性 遗传分析 分子定位 

分 类 号：S511[农业科学—作物学]