Predicted methylation landscape of all CpG islands on the human genome  

作　　者：FAN ShiCai ZOU JianXiao XU HongBing ZHANG XueGong 

机构地区：[1]School of Automation Engineering, University of Electronic Science and Technology of China, Chengdu 610054, China [2]MOE Key Laboratory of Bioinformatics and Bioinformatics Division, TNLIST/ Department of Automation, Tsinghua University, Beijing 100084, China

出　　处：《Chinese Science Bulletin》2010年第22期2353-2358,共6页

基　　金：supported by the National Natural Science Foundation of China (30625012 and 60721003);National Basic Research Program of China (2004CB518605)

摘　　要：CpG island methylation plays important role in various biological processes. To investigate methylation landscape of all CpG islands on the human genome, we develop a model for predicting the CpG island methylation status. This model outperforms other existing methods. We apply the model on the whole human genome and predict the landscape of DNA methylation of all CpG islands. Based on the methylation profile, we find that about 31% of CpG islands are methylation-prone and CpG islands located in promoter regions are seldom methylated. There is no significant difference in the CpG island methylation level between R and G bands among the chromosomes. The occupancy of RNA polymerase II is significantly higher in methylation-resistant promoter CpG islands, indicating that genes with such promoter CpG islands tend to be more active.CpG island methylation plays important role in various biological processes. To investigate methylation landscape of all CpG islands on the human genome, we develop a model for predicting the CpG island methylation status. This model outperforms other existing methods. We apply the model on the whole human genome and predict the landscape of DNA methylation of all CpG islands. Based on the methylation profile, we find that about 31% of CpG islands are methylation-prone and CpG islands located in promoter regions are seldom methylated. There is no significant difference in the CpG island methylation level between R and G bands among the chromosomes. The occupancy of RNA polymerase Ⅱ is significantly higher in methylation-resistant promoter CpG islands, indicating that genes with such promoter CpG islands tend to be more active.

关 键 词：DNA甲基化 人类基因组 CPG岛 景观 预测 RNA聚合酶 基因启动子 生物过程 

分 类 号：Q75[生物学—分子生物学] Q987